FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081738243> ?p ?o ?g. }

• W2081738243 endingPage "1426" @default.
• W2081738243 startingPage "1419" @default.
• W2081738243 abstract "A knowledge of beta cell-specific gene expression provides a basis for identifying proteins potentially involved in beta cell function and pathology. To identify candidate beta cell-specific genes, we applied the PCR-based subtractive hybridization technique of representational difference analysis (RDA) to the mouse SV40-transformed endocrine cell lines, betaTC3 and alphaTC1. Following three successive subtractions of alphaTC1 complementary DNA from betaTC3 complementary DNA, difference products were cloned into pUC19 and nucleotide sequences determined. Comparison of 91 sequences against the databases identified 11 known and 8 novel genes. Known genes included previously reported beta cell-specific genes, insulin I/II and islet amyloid polypeptide, as well as other non-beta cell-specific genes such as those for insulin-like growth factor II, selenoprotein P, neuronatin, prohormone convertase, and type 1 protein kinase A regulatory subunit. By Northern blot hybridization, expression of the majority of known and novel genes was restricted to betaTC3 cells. Novel genes BA-12, -13, -14, and -18 were expressed not only in betaTC3 cells, but also in normal pancreatic islets and a limited number of other tissues. The deduced amino acid sequence of BA-14 showed significant homology with members of the cadherin superfamily indicating that BA-14 may encode a cadherin-like molecule potentially involved in beta cell adhesion events during islet ontogeny. In betaTC3 cells, none of the novel genes were regulated at the RNA level by high glucose. However, in parallel studies, transcription of BA-12 was significantly increased by both sodium butyrate and nicotinamide, suggesting that this gene may play a role in pancreatic beta cell growth and/or differentiation. In this study, we have demonstrated that cRDA is an effective strategy for systematically mapping differences in gene expression between two related but functionally-distinct endocrine cells. Its application to experimental animal models of islet-cell regeneration may facilitate the discovery of potential factors that mediate beta cell growth and differentiation." @default.
• W2081738243 created "2016-06-24" @default.
• W2081738243 creator A5011807996 @default.
• W2081738243 creator A5049193991 @default.
• W2081738243 creator A5064530813 @default.
• W2081738243 creator A5069612841 @default.
• W2081738243 date "1997-04-01" @default.
• W2081738243 modified "2023-10-17" @default.
• W2081738243 title "Identification of Pancreatic β Cell-Related Genes by Representational Difference Analysis1" @default.
• W2081738243 cites W1495959646 @default.
• W2081738243 cites W1581203443 @default.
• W2081738243 cites W158610362 @default.
• W2081738243 cites W1620880263 @default.
• W2081738243 cites W1824007417 @default.
• W2081738243 cites W1971756446 @default.
• W2081738243 cites W1974068660 @default.
• W2081738243 cites W1977579221 @default.
• W2081738243 cites W1981752846 @default.
• W2081738243 cites W1981969181 @default.
• W2081738243 cites W1985916621 @default.
• W2081738243 cites W2004526841 @default.
• W2081738243 cites W2019561438 @default.
• W2081738243 cites W2023434101 @default.
• W2081738243 cites W2027590165 @default.
• W2081738243 cites W2027797226 @default.
• W2081738243 cites W2038862314 @default.
• W2081738243 cites W2051987305 @default.
• W2081738243 cites W2070972996 @default.
• W2081738243 cites W2071615189 @default.
• W2081738243 cites W2072652145 @default.
• W2081738243 cites W2076530564 @default.
• W2081738243 cites W2088130063 @default.
• W2081738243 cites W2091201090 @default.
• W2081738243 cites W2096192327 @default.
• W2081738243 cites W2106437575 @default.
• W2081738243 cites W2106490597 @default.
• W2081738243 cites W2123436766 @default.
• W2081738243 cites W2164998638 @default.
• W2081738243 cites W2171854519 @default.
• W2081738243 cites W59323898 @default.
• W2081738243 doi "https://doi.org/10.1210/endo.138.4.5066" @default.
• W2081738243 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9075697" @default.
• W2081738243 hasPublicationYear "1997" @default.
• W2081738243 type Work @default.
• W2081738243 sameAs 2081738243 @default.
• W2081738243 citedByCount "38" @default.
• W2081738243 countsByYear W20817382432013 @default.
• W2081738243 countsByYear W20817382432014 @default.
• W2081738243 countsByYear W20817382432016 @default.
• W2081738243 countsByYear W20817382432017 @default.
• W2081738243 countsByYear W20817382432018 @default.
• W2081738243 countsByYear W20817382432020 @default.
• W2081738243 countsByYear W20817382432022 @default.
• W2081738243 countsByYear W20817382432023 @default.
• W2081738243 crossrefType "journal-article" @default.
• W2081738243 hasAuthorship W2081738243A5011807996 @default.
• W2081738243 hasAuthorship W2081738243A5049193991 @default.
• W2081738243 hasAuthorship W2081738243A5064530813 @default.
• W2081738243 hasAuthorship W2081738243A5069612841 @default.
• W2081738243 hasBestOaLocation W20817382431 @default.
• W2081738243 hasConcept C103395026 @default.
• W2081738243 hasConcept C104317684 @default.
• W2081738243 hasConcept C121587040 @default.
• W2081738243 hasConcept C134018914 @default.
• W2081738243 hasConcept C150194340 @default.
• W2081738243 hasConcept C153911025 @default.
• W2081738243 hasConcept C165220095 @default.
• W2081738243 hasConcept C189482833 @default.
• W2081738243 hasConcept C191370860 @default.
• W2081738243 hasConcept C196436469 @default.
• W2081738243 hasConcept C2776828364 @default.
• W2081738243 hasConcept C2779306644 @default.
• W2081738243 hasConcept C2779372761 @default.
• W2081738243 hasConcept C46699223 @default.
• W2081738243 hasConcept C54355233 @default.
• W2081738243 hasConcept C55493867 @default.
• W2081738243 hasConcept C71315377 @default.
• W2081738243 hasConcept C86803240 @default.
• W2081738243 hasConceptScore W2081738243C103395026 @default.
• W2081738243 hasConceptScore W2081738243C104317684 @default.
• W2081738243 hasConceptScore W2081738243C121587040 @default.
• W2081738243 hasConceptScore W2081738243C134018914 @default.
• W2081738243 hasConceptScore W2081738243C150194340 @default.
• W2081738243 hasConceptScore W2081738243C153911025 @default.
• W2081738243 hasConceptScore W2081738243C165220095 @default.
• W2081738243 hasConceptScore W2081738243C189482833 @default.
• W2081738243 hasConceptScore W2081738243C191370860 @default.
• W2081738243 hasConceptScore W2081738243C196436469 @default.
• W2081738243 hasConceptScore W2081738243C2776828364 @default.
• W2081738243 hasConceptScore W2081738243C2779306644 @default.
• W2081738243 hasConceptScore W2081738243C2779372761 @default.
• W2081738243 hasConceptScore W2081738243C46699223 @default.
• W2081738243 hasConceptScore W2081738243C54355233 @default.
• W2081738243 hasConceptScore W2081738243C55493867 @default.
• W2081738243 hasConceptScore W2081738243C71315377 @default.
• W2081738243 hasConceptScore W2081738243C86803240 @default.
• W2081738243 hasIssue "4" @default.
• W2081738243 hasLocation W20817382431 @default.

• next