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• W2081743980 abstract "En el campo de la identificación humana, la genética forense está teniendo un papel relevante en los últimos años. Sin embargo, existen circunstancias en las que su labor puede verse dificultada. Tal es el caso del hallazgo de restos humanos esqueletizados, en los que las condiciones tafonómicas de dicho hallazgo pueden suponer una limitación en la obtención de ADN a partir de ellos en cantidad y calidad suficiente para su identificación. En estos casos se han de aplicar estrategias especiales y diferentes a las habitualmente empleadas en los laboratorios forenses. En la presente revisión, en primer lugar, se valora la idoneidad del uso de restos óseos para la obtención de ADN, que será empleado en los subsiguientes estudios genéticos de identificación. En segundo lugar, se comentan los problemas de preservación del ADN obtenido a partir de este tipo de muestras. Igualmente se exponen las complicaciones metodológicas que implica la obtención de este ADN. Aunque no debe olvidarse la degradación y las modificaciones moleculares de este tipo de ADN, la presente revisión se centra en su coextracción junto con inhibidores de la PCR. Finalmente, se valoran los principales protocolos de extracción de ADN a partir de restos óseos, encaminados precisamente a la eliminación de dichos inhibidores. In the human identification field, Forensic Genetics serves an outstanding role every time. However, there are circumstances where its task could be obstructed. This is the case of human dried bones discovery. The taphonomic conditions of this finding could imply a limitation on adequate quantity and quality of obtaining DNA for bone identification. In these cases, special and different strategies to the usual ones must be executed on forensic laboratories. In the present revision, firstly, the adequacy of using bone fragments to obtain DNA is assessed. This extracted DNA will be employed on subsequent identification genetic studies. Secondly, preservation problems of DNA obtained from this type of samples will be presented. Likewise, methodological complications to obtain this DNA will be set out. In particular, the co-extraction with PCR inhibitors will be considered, although degradation and molecular modification of DNA must not be forgotten. Finally, principal DNA extraction protocols of bone fragments will be evaluated. The protocols, which are designed to eliminate those PCR inhibitors, will be especially considered." @default.
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• W2081743980 date "2013-04-01" @default.
• W2081743980 modified "2023-10-05" @default.
• W2081743980 title "Revisión de métodos de extracción de ADN a partir de restos óseos en el laboratorio forense" @default.
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