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• W2081784372 abstract "Surgical procedures as a prelude to optical imaging are a rate-limiting step in experimental neuroscience. Towards automation of these procedures, we describe the use of nonlinear optical techniques to create a thinned skull window for transcranial imaging. Metrology by second harmonic generation was used to map the surfaces of the skull and define a cutting path. Plasma-mediated laser ablation was utilized to cut bone. Mice prepared with these techniques were used to image subsurface cortical vasculature and blood flow. The viability of the brain tissue was confirmed via histological analysis and supports the utility of solely optical techniques for osteotomy and potentially other surgical procedures." @default.
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• W2081784372 date "2013-09-24" @default.
• W2081784372 modified "2023-10-03" @default.
• W2081784372 title "All-optical osteotomy to create windows for transcranial imaging in mice" @default.
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• W2081784372 doi "https://doi.org/10.1364/oe.21.023160" @default.
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