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- W2081825701 abstract "Nicotine biosynthesis in tobacco callus tissues was investigated by comparing high nicotine producing strain OMT-53 with scarcely producing strain NT–5. NT–5 strain which had been subcultured for about 5 years with a medium containing 1.0 ppm 2,4-D, produced very little amount of nicotine even after more than 47 subcultures on OT–23 medium containing 0.15 ppm α-NAA. On the other hand, OMT–53 strain maintained a constant high level nicotine producing (ca. 2% or more on D.W. basis) capacity over 50 successive cultures on the same medium. The growth response of NT–5 callus tissues to α-NAA concentration was quite different from that of OMT–53. Nicotine production assumed more distinctive patterns with these two strains. NT–5 strain never produced nicotine at any concentrations of α-NAA examined, whilst OMT–53 strain produced abundant amount of nicotine within a narrow range of α-NAA concentration having the optimal value of 0.15 ppm. Nicotine supplemented to culture medium could not be decomposed either with..." @default.
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- W2081825701 date "1978-01-01" @default.
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- W2081825701 title "Some accounts of nicotine biosynthesis in tobacco callus tissues by the use of effective and ineffective strains." @default.
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- W2081825701 doi "https://doi.org/10.1271/bbb1961.42.1733" @default.
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