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- W2081849062 abstract "Various methods generally used for isolation and fractionation of mouse liver nuclei are reviewed by electron microscopic examination and biochemical estimations. All methods previously employed yield nuclei which are not sufficiently stabilized to enable correct subfractionation. During isolation and fractionation, nuclear components undergo structural modifications which lead to false separation of euchromatin and heterochromatin. A method was developed to enable true separation of these two fractions of chromatin. Mouse liver nuclei are prepared according to Chauveau's procedure in 2.2 M sucrose containing Tris 1 mM pH 7.5, 25 mM KCl, CaCl2 and MgCl2 0.9 mM each and 0.14 mM spermidine. After sonication, nuclear components are then recovered by differential centrifugation according to a modification of Frenster's technique. Three groups of fractions are obtained: a heavy fraction (fractions I and II of Frenster) which is mainly composed of nucleoli, nucleolus-associated chromatin and heterochromatin; an intermediate fraction (fraction III) obtained by centrifugation at 78 000 g for 1 h and composed of nuclear membranes and particles; a light fraction (fraction IV) which corresponds to euchromatin and which can be collected by centrifugation at 105 000 g for 18 h. Further purification of these nuclear subfractions is now being investigated." @default.
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- W2081849062 date "1973-11-01" @default.
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- W2081849062 title "Aspects structuraux et biochimiques des effets de quelques milieux utilises pour l'isolement et le fractionnement du noyau de foie de souris" @default.
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- W2081849062 doi "https://doi.org/10.1016/0014-4827(73)90238-3" @default.
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