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- W2082051236 abstract "Optimum conditions were selected for testing of direct and indirect H-2 reactive rosette-forming cells (RFC) with the use of sheep or mouse erythrocytes coated with soluble H-2 antigens. Rosette formation with mouse erythrocytes is shown to be an immunologically specific reaction and to be inhibited by the pretreatment of cells with the corresponding soluble H-2 antigen preparation. Rate of killer cell and RFC formation is different after primary and secondary immunization with an allogeneic tumor, Unlike killer cells. RFCs are inactivated without complement by rabbit antibodies against mouse γ-globulin (MGG). In contrast to killer cells, which are eliminated in the presence of complement by anti-θ antibodies but not by anti-plasma cell antigen (PC.1) and anti-MGG antibodies, direct RFCs are not inactivated by anti-θ but are eliminated partiallv by anti-MGG and anti-PC1. The RFCs are shown to be concentrated in the low-density fractions of the discontinuous bovine serum albumin gradient, whereas cytotoxic lymphocytes are distributed less closely, being concentrated in the intermediate fraction, which does not differ morphologically from the initial cell suspension. Killer and rosette-forming cells reactive to the same H-2 antigens appear to be two non-overlapping populations of T and B cells, respectively, and no T-B cooperation is required for destruction of target cells." @default.
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- W2082051236 date "1973-11-01" @default.
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- W2082051236 title "Received 14 May 1973; Relationship between Killer and Rosette-Forming Cells Reactive to H-2 478" @default.
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- W2082051236 doi "https://doi.org/10.1111/j.1365-3083.1973.tb02054.x" @default.
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