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• W2082052521 abstract "It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site–leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes." @default.
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• W2082052521 date "2015-03-17" @default.
• W2082052521 modified "2023-10-10" @default.
• W2082052521 title "Development of molecular markers tightly linked to Pvr4 gene in pepper using next-generation sequencing" @default.
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• W2082052521 doi "https://doi.org/10.1007/s11032-015-0294-5" @default.
• W2082052521 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4361654" @default.
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