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- W2082057231 abstract "Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) followed by immunoblotting was employed to detect intracellular precursors of endo-β-1,4-glucanases (EGs) in Trichoderma reesei QM9414 under conditions of de novo induction by sophorose and de novo carbon catabolite derepression by lactose. Secretion of EGs was always preceded by intracellular accumulation of lower Mr precursors, which became processed to larger Mr forms immediately prior to their extracellular appearance. Treatment of the larger Mr forms with α-mannosidase converted them to forms with the same Mr as the smaller forms, whereas Endo H treatment was without effect. These results are consistent with a requirement of O-linked glycosylation for secretion of EGs by T. reesei." @default.
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- W2082057231 date "1988-05-01" @default.
- W2082057231 modified "2023-09-27" @default.
- W2082057231 title "Intracellular precursors of endo-β-1,4-glucanase inTrichoderma reesei" @default.
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- W2082057231 doi "https://doi.org/10.1111/j.1574-6968.1988.tb02943.x" @default.
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