FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082138600> ?p ?o ?g. }

• W2082138600 abstract "Mitochondrial Hsp70 (mtHsp70) is essential for a vast repertoire of functions, including protein import, and requires effective interdomain communication for efficient partner-protein interactions. However, the in vivo functional significance of allosteric regulation in eukaryotes is poorly defined. Using integrated biochemical and yeast genetic approaches, we provide compelling evidence that a conserved substrate-binding domain (SBD) loop, L 4,5 , plays a critical role in allosteric communication governing mtHsp70 chaperone functions across species. In yeast, a temperature-sensitive L 4,5 mutation (E467A) disrupts bidirectional domain communication, leading to compromised protein import and mitochondrial function. Loop L 4,5 functions synergistically with the linker in modulating the allosteric interface and conformational transitions between SBD and the nucleotide-binding domain (NBD), thus regulating interdomain communication. Second-site intragenic suppressors of E467A isolated within the SBD suppress domain communication defects by conformationally altering the allosteric interface, thereby restoring import and growth phenotypes. Strikingly, the suppressor mutations highlight that restoration of communication from NBD to SBD alone is the minimum essential requirement for effective in vivo function when primed at higher basal ATPase activity, mimicking the J-protein–bound state. Together these findings provide the first mechanistic insights into critical regions within the SBD of mtHsp70s regulating interdomain communication, thus highlighting its importance in protein translocation and mitochondrial biogenesis." @default.
• W2082138600 created "2016-06-24" @default.
• W2082138600 creator A5011136081 @default.
• W2082138600 creator A5016194841 @default.
• W2082138600 creator A5023767014 @default.
• W2082138600 creator A5046193774 @default.
• W2082138600 creator A5063411959 @default.
• W2082138600 date "2014-07-15" @default.
• W2082138600 modified "2023-09-26" @default.
• W2082138600 title "Role of the loop L4,5 in allosteric regulation in mtHsp70s: in vivo significance of domain communication and its implications in protein translocation" @default.
• W2082138600 cites W1498255154 @default.
• W2082138600 cites W1591848571 @default.
• W2082138600 cites W1964995688 @default.
• W2082138600 cites W1967960279 @default.
• W2082138600 cites W1976476593 @default.
• W2082138600 cites W1977385837 @default.
• W2082138600 cites W1981188702 @default.
• W2082138600 cites W1989189288 @default.
• W2082138600 cites W1991581560 @default.
• W2082138600 cites W1991970381 @default.
• W2082138600 cites W1992659479 @default.
• W2082138600 cites W1996623614 @default.
• W2082138600 cites W1999157304 @default.
• W2082138600 cites W1999268424 @default.
• W2082138600 cites W1999778386 @default.
• W2082138600 cites W2010009570 @default.
• W2082138600 cites W2014294043 @default.
• W2082138600 cites W2018523815 @default.
• W2082138600 cites W2020220420 @default.
• W2082138600 cites W2023953172 @default.
• W2082138600 cites W2025906616 @default.
• W2082138600 cites W2030438778 @default.
• W2082138600 cites W2036794157 @default.
• W2082138600 cites W2040795144 @default.
• W2082138600 cites W2042838776 @default.
• W2082138600 cites W2043693325 @default.
• W2082138600 cites W2053544172 @default.
• W2082138600 cites W2055269320 @default.
• W2082138600 cites W2064123540 @default.
• W2082138600 cites W2064676619 @default.
• W2082138600 cites W2072550162 @default.
• W2082138600 cites W2073580695 @default.
• W2082138600 cites W2077403816 @default.
• W2082138600 cites W2081106008 @default.
• W2082138600 cites W2085440025 @default.
• W2082138600 cites W2086922403 @default.
• W2082138600 cites W2109936231 @default.
• W2082138600 cites W2111846511 @default.
• W2082138600 cites W2114126301 @default.
• W2082138600 cites W2116069733 @default.
• W2082138600 cites W2117785727 @default.
• W2082138600 cites W2141002664 @default.
• W2082138600 cites W2150094763 @default.
• W2082138600 cites W2150164439 @default.
• W2082138600 cites W2157926893 @default.
• W2082138600 cites W2161357377 @default.
• W2082138600 cites W2162937818 @default.
• W2082138600 cites W2166153628 @default.
• W2082138600 cites W2166664098 @default.
• W2082138600 cites W2168009822 @default.
• W2082138600 cites W2270651270 @default.
• W2082138600 cites W82016731 @default.
• W2082138600 doi "https://doi.org/10.1091/mbc.e14-03-0821" @default.
• W2082138600 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4091826" @default.
• W2082138600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24829379" @default.
• W2082138600 hasPublicationYear "2014" @default.
• W2082138600 type Work @default.
• W2082138600 sameAs 2082138600 @default.
• W2082138600 citedByCount "1" @default.
• W2082138600 countsByYear W20821386002015 @default.
• W2082138600 crossrefType "journal-article" @default.
• W2082138600 hasAuthorship W2082138600A5011136081 @default.
• W2082138600 hasAuthorship W2082138600A5016194841 @default.
• W2082138600 hasAuthorship W2082138600A5023767014 @default.
• W2082138600 hasAuthorship W2082138600A5046193774 @default.
• W2082138600 hasAuthorship W2082138600A5063411959 @default.
• W2082138600 hasBestOaLocation W20821386001 @default.
• W2082138600 hasConcept C104317684 @default.
• W2082138600 hasConcept C11804247 @default.
• W2082138600 hasConcept C131934819 @default.
• W2082138600 hasConcept C14036430 @default.
• W2082138600 hasConcept C142724271 @default.
• W2082138600 hasConcept C144292202 @default.
• W2082138600 hasConcept C166342909 @default.
• W2082138600 hasConcept C170493617 @default.
• W2082138600 hasConcept C2775962898 @default.
• W2082138600 hasConcept C51639874 @default.
• W2082138600 hasConcept C54355233 @default.
• W2082138600 hasConcept C71924100 @default.
• W2082138600 hasConcept C86803240 @default.
• W2082138600 hasConcept C95444343 @default.
• W2082138600 hasConceptScore W2082138600C104317684 @default.
• W2082138600 hasConceptScore W2082138600C11804247 @default.
• W2082138600 hasConceptScore W2082138600C131934819 @default.
• W2082138600 hasConceptScore W2082138600C14036430 @default.
• W2082138600 hasConceptScore W2082138600C142724271 @default.
• W2082138600 hasConceptScore W2082138600C144292202 @default.
• W2082138600 hasConceptScore W2082138600C166342909 @default.
• W2082138600 hasConceptScore W2082138600C170493617 @default.
• W2082138600 hasConceptScore W2082138600C2775962898 @default.

• next