Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082144779> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2082144779 endingPage "6" @default.
- W2082144779 startingPage "1" @default.
- W2082144779 abstract "Concensus primers designed to CYP1A-conserved regions were used to amplify a 1.3 kb probe from flounder genomic DNA via polymerase chain reaction (PCR). A 14-kb clone was isolated from a flounder genomic library constructed in lambda FIXII. Of this clone, 8 kb was sequenced, including 3 kb of upstream sequence. The predicted amino acid sequence showed closest similarity to plaice CYP1A1 (98%). Gene structure conformed to the seven exons and six introns common to previous CYP1A sequences, but intron lengths were not conserved. Concensus sequences corresponding to xenobiotic and other response elements as well as TATA, CAAT and GC boxes were identified. Upstream sequence (3.5 kb) including the first exon and intron up to the putative start codon were amplified via PCR and inserted upstream of the luciferase gene in a pGL3 reporter gene construct. The HepG2 mammalian hepatoma cell line was transiently co-transfected with the flounder CYP1A reporter gene construct and the pRL-CMV internal control construct. The maximal induction upon exposure to 100 nM 3-MC was 4.4-fold in comparison with carrier-treated cells. Use of deletion constructs resulted in loss of inducibility." @default.
- W2082144779 created "2016-06-24" @default.
- W2082144779 creator A5027095776 @default.
- W2082144779 creator A5030300554 @default.
- W2082144779 creator A5071948105 @default.
- W2082144779 creator A5089666883 @default.
- W2082144779 date "2000-07-01" @default.
- W2082144779 modified "2023-10-18" @default.
- W2082144779 title "The cytochrome P450 1A gene (CYP1A) from European flounder (Platichthys flesus), analysis of regulatory regions and development of a dual luciferase reporter gene system" @default.
- W2082144779 cites W1588868156 @default.
- W2082144779 cites W1964198497 @default.
- W2082144779 cites W1986767179 @default.
- W2082144779 cites W1994623914 @default.
- W2082144779 cites W1997112703 @default.
- W2082144779 cites W2008850586 @default.
- W2082144779 cites W2044317964 @default.
- W2082144779 cites W2103552637 @default.
- W2082144779 cites W4256006582 @default.
- W2082144779 doi "https://doi.org/10.1016/s0141-1136(00)00053-2" @default.
- W2082144779 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11460673" @default.
- W2082144779 hasPublicationYear "2000" @default.
- W2082144779 type Work @default.
- W2082144779 sameAs 2082144779 @default.
- W2082144779 citedByCount "47" @default.
- W2082144779 countsByYear W20821447792012 @default.
- W2082144779 countsByYear W20821447792013 @default.
- W2082144779 countsByYear W20821447792014 @default.
- W2082144779 countsByYear W20821447792015 @default.
- W2082144779 countsByYear W20821447792016 @default.
- W2082144779 countsByYear W20821447792018 @default.
- W2082144779 countsByYear W20821447792020 @default.
- W2082144779 countsByYear W20821447792022 @default.
- W2082144779 crossrefType "journal-article" @default.
- W2082144779 hasAuthorship W2082144779A5027095776 @default.
- W2082144779 hasAuthorship W2082144779A5030300554 @default.
- W2082144779 hasAuthorship W2082144779A5071948105 @default.
- W2082144779 hasAuthorship W2082144779A5089666883 @default.
- W2082144779 hasConcept C101762097 @default.
- W2082144779 hasConcept C104317684 @default.
- W2082144779 hasConcept C111335760 @default.
- W2082144779 hasConcept C150194340 @default.
- W2082144779 hasConcept C153911025 @default.
- W2082144779 hasConcept C161733203 @default.
- W2082144779 hasConcept C17757408 @default.
- W2082144779 hasConcept C2776367567 @default.
- W2082144779 hasConcept C2909208804 @default.
- W2082144779 hasConcept C36823959 @default.
- W2082144779 hasConcept C505870484 @default.
- W2082144779 hasConcept C54009773 @default.
- W2082144779 hasConcept C54355233 @default.
- W2082144779 hasConcept C65888428 @default.
- W2082144779 hasConcept C86803240 @default.
- W2082144779 hasConcept C94671646 @default.
- W2082144779 hasConceptScore W2082144779C101762097 @default.
- W2082144779 hasConceptScore W2082144779C104317684 @default.
- W2082144779 hasConceptScore W2082144779C111335760 @default.
- W2082144779 hasConceptScore W2082144779C150194340 @default.
- W2082144779 hasConceptScore W2082144779C153911025 @default.
- W2082144779 hasConceptScore W2082144779C161733203 @default.
- W2082144779 hasConceptScore W2082144779C17757408 @default.
- W2082144779 hasConceptScore W2082144779C2776367567 @default.
- W2082144779 hasConceptScore W2082144779C2909208804 @default.
- W2082144779 hasConceptScore W2082144779C36823959 @default.
- W2082144779 hasConceptScore W2082144779C505870484 @default.
- W2082144779 hasConceptScore W2082144779C54009773 @default.
- W2082144779 hasConceptScore W2082144779C54355233 @default.
- W2082144779 hasConceptScore W2082144779C65888428 @default.
- W2082144779 hasConceptScore W2082144779C86803240 @default.
- W2082144779 hasConceptScore W2082144779C94671646 @default.
- W2082144779 hasIssue "1-5" @default.
- W2082144779 hasLocation W20821447791 @default.
- W2082144779 hasLocation W20821447792 @default.
- W2082144779 hasOpenAccess W2082144779 @default.
- W2082144779 hasPrimaryLocation W20821447791 @default.
- W2082144779 hasRelatedWork W1692571807 @default.
- W2082144779 hasRelatedWork W1960333619 @default.
- W2082144779 hasRelatedWork W2063119860 @default.
- W2082144779 hasRelatedWork W2089158163 @default.
- W2082144779 hasRelatedWork W2099193831 @default.
- W2082144779 hasRelatedWork W2397667500 @default.
- W2082144779 hasRelatedWork W2415371082 @default.
- W2082144779 hasRelatedWork W260640041 @default.
- W2082144779 hasRelatedWork W3125147123 @default.
- W2082144779 hasRelatedWork W83646335 @default.
- W2082144779 hasVolume "50" @default.
- W2082144779 isParatext "false" @default.
- W2082144779 isRetracted "false" @default.
- W2082144779 magId "2082144779" @default.
- W2082144779 workType "article" @default.