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• W2082148336 abstract "This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young’s modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (Linstantaneous) and transitional aspiration length (Ltransitional), which was further translated to Einstantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine Cspecific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing Einstantaneous of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular Einstantaneous and Cspecific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties." @default.
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• W2082148336 date "2015-01-27" @default.
• W2082148336 modified "2023-10-11" @default.
• W2082148336 title "Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System" @default.
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• W2082148336 doi "https://doi.org/10.3390/s150202763" @default.
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