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- W2082148536 abstract "This paper describes a reductive amination crosslinking protocol that facilitates identification and characterization of a class of DNA repair enzymes, DNA glycosylase/AP lyases, which are involved in base excision repair. This crosslinking technique has been used to identify enzymes in crude extracts and in partially purified enzyme preparations, to isolate proteins for sequencing, and to confirm the reaction mechanism of members of this enzyme family. Chemical reduction of the Schiff's base enzyme-substrate intermediate to a stable amine results in the formation of an irreversible covalent bond between the substrate lesion situated within a 2'-deoxyoligonucleotide and the repair enzyme. This complex can be detected by gel electrophoresis and can also be isolated and analyzed by amino acid sequencing." @default.
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- W2082148536 date "2000-10-01" @default.
- W2082148536 modified "2023-09-30" @default.
- W2082148536 title "Identification, Characterization, and Purification of DNA Glycosylase/AP Lyases by Reductive Crosslinking to 2′-Deoxyribooligonucleotides Containing Specific Base Lesions" @default.
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- W2082148536 doi "https://doi.org/10.1006/meth.2000.1059" @default.
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