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- W2082171608 abstract "A system has been developed for the biochemical study of male pronuclear histones following fertilization. Pronuclei are isolated in high yield and purity from polyspermically fertilized sea urchin eggs. Acid extracts of the pronuclei or chromatin are analyzed by two-dimensional gel electrophoresis. During male pronuclear decondensation, sperm-specific (Sp) H1 is completely replaced by the cleavage-stage (CS) H1 variant and two sperm H2B species are more gradually replaced by two closely migrating species (O, P) perhaps modifications of the Sp2Bs. Histones CS2A and CS2B begin to accumulate on the male chromatin predominantly during DNA synthesis, as does a subspecies of H3 (3″). By the time of chromosome condensation, the histone complement is chiefly CS1; CS2A and Sp2A; CS2B and O, P; three H3s and two H4s. Controls indicate that these patterns are not artifacts of cytoplasmic or maternal pronuclear contamination or modification of sperm histones during isolation. The histone transitions occur even if protein synthesis is inhibited, suggesting a maternal pool of CS histones. Such a store can be demonstrated directly in acid extracts of whole eggs. At least 30 haploid equivalents of CS histones are stored per egg. No α, β, γ, or δ variants appear on the chromatin in the first cycle. This system should allow, for the first time, detailed biochemical study of male pronuclear chromatin." @default.
- W2082171608 created "2016-06-24" @default.
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- W2082171608 date "1981-03-01" @default.
- W2082171608 modified "2023-10-18" @default.
- W2082171608 title "Transitions in histone variants of the male pronucleus following fertilization and evidence for a maternal store of cleavage-stage histones in the sea urchin egg" @default.
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- W2082171608 doi "https://doi.org/10.1016/0012-1606(81)90452-8" @default.
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