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- W2082256013 abstract "A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNA(Met) sample and threonylated to 76% of the level observed with native tRNA(fMet) from E. coli." @default.
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- W2082256013 date "1990-08-20" @default.
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- W2082256013 title "Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNAMet" @default.
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- W2082256013 doi "https://doi.org/10.1016/0014-5793(90)81118-8" @default.
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