FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082269897> ?p ?o ?g. }

• W2082269897 endingPage "48" @default.
• W2082269897 startingPage "39" @default.
• W2082269897 abstract "Growth of cultured cells in low potassium medium has been shown to result in an increase in the number of Na,K-ATPase sites. This phenomenon and its physiological and pharmacological consequences were examined in spontaneously beating monolayers of cultured chick heart cells. Growth of cells in 1 mM extracellular potassium, 2 microM ouabain, or 1 microM veratridine for 48 hours caused 60%, 40%, or 20% increases, respectively, in the total number of specific ouabain binding sites measurable in intact cells. Acute exposure of control cells grown in 4 mM to 1 mM extracellular potassium caused elevation of steady state [Na+]i by 37%, while 1 microM veratridine exposure increased [Na+]i by 12%. After 48 hours of growth in 1 mM extracellular potassium, intracellular sodium concentrations declined to near-control levels. In cells grown in low extracellular potassium and then equilibrated with 4 mM potassium for 30 minutes, the positive inotropic effects of 1 mM extracellular potassium and 0.3 microM isoproterenol, expressed as a percent of contractile response to 3.6 mM calcium, were 40 +/- 6% and 37 +/- 5% (means +/- SEM), respectively, in low potassium-grown cells, compared with 63 +/- 8% and 35 +/- 4% in control cells. Growth of cells in low potassium shifted the concentration-effect curve for ouabain to the right. The rapid component of calcium uptake in zero extracellular sodium was significantly lower in low potassium-grown cells than in control cells after equilibration in 1 mM extracellular potassium for 30 minutes. These findings demonstrate that prolonged exposure of cultured heart cells to 1 mM extracellular potassium or ouabain causes induction of additional functional sarcolemmal sodium pump sites. The increased levels of intracellular sodium caused by these interventions appear to be an important determinant of sodium pump site density. The reduced contractile response of cells grown in 1 mM extracellular potassium and ouabain (but not isoproterenol) supports the view that elevated intracellular sodium due to Na,K-ATPase inhibition mediates the positive inotropic response to low extracellular potassium and ouabain, probably via augmented transsarcolemmal sodium-calcium exchange. In addition, our results support a mechanism of inotropic action of digitalis glycosides based on inhibition of the sodium pump rather than altered calcium binding properties of sarcolemmal sites due to cardiac glycoside binding to Na,K-ATPase." @default.
• W2082269897 created "2016-06-24" @default.
• W2082269897 creator A5006718419 @default.
• W2082269897 creator A5060225883 @default.
• W2082269897 creator A5064305448 @default.
• W2082269897 creator A5082857119 @default.
• W2082269897 date "1984-07-01" @default.
• W2082269897 modified "2023-09-25" @default.
• W2082269897 title "Effects of growth in low potassium medium or ouabain on membrane Na,K-ATPase, cation transport, and contractility in cultured chick heart cells." @default.
• W2082269897 cites W1775749144 @default.
• W2082269897 cites W1849732852 @default.
• W2082269897 cites W1975089277 @default.
• W2082269897 cites W1975627597 @default.
• W2082269897 cites W2005183497 @default.
• W2082269897 cites W2010983615 @default.
• W2082269897 cites W2020257945 @default.
• W2082269897 cites W2028305348 @default.
• W2082269897 cites W2030781430 @default.
• W2082269897 cites W2045591004 @default.
• W2082269897 cites W2048347267 @default.
• W2082269897 cites W2059430954 @default.
• W2082269897 cites W2063057507 @default.
• W2082269897 cites W2064551974 @default.
• W2082269897 cites W2068955622 @default.
• W2082269897 cites W2077172331 @default.
• W2082269897 cites W2085410855 @default.
• W2082269897 cites W2092071834 @default.
• W2082269897 cites W2093323095 @default.
• W2082269897 cites W2157766074 @default.
• W2082269897 cites W2160357702 @default.
• W2082269897 cites W2165248369 @default.
• W2082269897 cites W2174405355 @default.
• W2082269897 cites W2205753780 @default.
• W2082269897 cites W2434182073 @default.
• W2082269897 doi "https://doi.org/10.1161/01.res.55.1.39" @default.
• W2082269897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6086172" @default.
• W2082269897 hasPublicationYear "1984" @default.
• W2082269897 type Work @default.
• W2082269897 sameAs 2082269897 @default.
• W2082269897 citedByCount "54" @default.
• W2082269897 crossrefType "journal-article" @default.
• W2082269897 hasAuthorship W2082269897A5006718419 @default.
• W2082269897 hasAuthorship W2082269897A5060225883 @default.
• W2082269897 hasAuthorship W2082269897A5064305448 @default.
• W2082269897 hasAuthorship W2082269897A5082857119 @default.
• W2082269897 hasBestOaLocation W20822698971 @default.
• W2082269897 hasConcept C12554922 @default.
• W2082269897 hasConcept C126322002 @default.
• W2082269897 hasConcept C134018914 @default.
• W2082269897 hasConcept C178790620 @default.
• W2082269897 hasConcept C185592680 @default.
• W2082269897 hasConcept C2776986425 @default.
• W2082269897 hasConcept C2779133857 @default.
• W2082269897 hasConcept C28406088 @default.
• W2082269897 hasConcept C39133596 @default.
• W2082269897 hasConcept C50952357 @default.
• W2082269897 hasConcept C517785266 @default.
• W2082269897 hasConcept C519063684 @default.
• W2082269897 hasConcept C537181965 @default.
• W2082269897 hasConcept C55493867 @default.
• W2082269897 hasConcept C71924100 @default.
• W2082269897 hasConcept C79879829 @default.
• W2082269897 hasConcept C83743174 @default.
• W2082269897 hasConcept C86803240 @default.
• W2082269897 hasConceptScore W2082269897C12554922 @default.
• W2082269897 hasConceptScore W2082269897C126322002 @default.
• W2082269897 hasConceptScore W2082269897C134018914 @default.
• W2082269897 hasConceptScore W2082269897C178790620 @default.
• W2082269897 hasConceptScore W2082269897C185592680 @default.
• W2082269897 hasConceptScore W2082269897C2776986425 @default.
• W2082269897 hasConceptScore W2082269897C2779133857 @default.
• W2082269897 hasConceptScore W2082269897C28406088 @default.
• W2082269897 hasConceptScore W2082269897C39133596 @default.
• W2082269897 hasConceptScore W2082269897C50952357 @default.
• W2082269897 hasConceptScore W2082269897C517785266 @default.
• W2082269897 hasConceptScore W2082269897C519063684 @default.
• W2082269897 hasConceptScore W2082269897C537181965 @default.
• W2082269897 hasConceptScore W2082269897C55493867 @default.
• W2082269897 hasConceptScore W2082269897C71924100 @default.
• W2082269897 hasConceptScore W2082269897C79879829 @default.
• W2082269897 hasConceptScore W2082269897C83743174 @default.
• W2082269897 hasConceptScore W2082269897C86803240 @default.
• W2082269897 hasIssue "1" @default.
• W2082269897 hasLocation W20822698971 @default.
• W2082269897 hasLocation W20822698972 @default.
• W2082269897 hasOpenAccess W2082269897 @default.
• W2082269897 hasPrimaryLocation W20822698971 @default.
• W2082269897 hasRelatedWork W1469370412 @default.
• W2082269897 hasRelatedWork W2042294732 @default.
• W2082269897 hasRelatedWork W2044406485 @default.
• W2082269897 hasRelatedWork W2076188683 @default.
• W2082269897 hasRelatedWork W2111321714 @default.
• W2082269897 hasRelatedWork W2162032112 @default.
• W2082269897 hasRelatedWork W2336389055 @default.
• W2082269897 hasRelatedWork W2428387744 @default.
• W2082269897 hasRelatedWork W1993656166 @default.
• W2082269897 hasRelatedWork W2012374663 @default.
• W2082269897 hasVolume "55" @default.

• next