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• W2082276468 abstract "Our laboratory demonstrated that endoplasmic reticulum iPLA2 (ER-iPLA2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA2 isoform(s) responsible for ER-iPLA2 activity in different species and tissues. ER-iPLA2 activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA2γ (group VIB PLA2) message in all tissues tested. Immunoblot analysis and PLA2 inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA2 in rabbit kidney and heart and rat kidney is iPLA2γ. These results demonstrate the expression of ER-iPLA2γ (group VIB) across species and tissues, and suggest that iPLA2γ may play critical roles in oxidant-induced cell injury." @default.
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• W2082276468 date "2005-02-01" @default.
• W2082276468 modified "2023-10-16" @default.
• W2082276468 title "Identification and distribution of endoplasmic reticulum iPLA2" @default.
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• W2082276468 doi "https://doi.org/10.1016/j.bbrc.2004.12.016" @default.
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