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• W2082319819 abstract "Hydrogen (H2) is a possible future alternative to current fossil-based transportation fuels; however, its lower explosive limit in air requires a reliable sensor to detect leaks wherever H2 is produced, stored, or used. Most current H2 sensors employ palladium or its alloy as the sensing element, featuring high operating temperature and limited selectivity. In this study, we report using soluble hydrogenase (SH) of aerobic beta-proteobacterium Ralstonia eutropha strain H16 to accomplish ambient, electrochemical detection of H2. Gas samples were collected in a solution containing SH that catalyzed the oxidation of H2. The electrons released during the H2 oxidation reaction were accepted by benzyl viologen (BV2+). The product of the redox reaction, BV+, was then detected using chronoamperometry. Using this sensing scheme, we demonstrate detection of H2 ranging from 1 to 100%. In addition, enzyme kinetics and the effect of oxygen on signal response were studied. Our results indicate that it is feasible to develop a sensor to detect H2 in the atmosphere that is based on enzyme-catalyzed electrochemical detection." @default.
• W2082319819 created "2016-06-24" @default.
• W2082319819 creator A5006549777 @default.
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• W2082319819 date "2005-06-11" @default.
• W2082319819 modified "2023-10-16" @default.
• W2082319819 title "Hydrogen Sensing by Enzyme-Catalyzed Electrochemical Detection" @default.
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• W2082319819 doi "https://doi.org/10.1021/ac050313i" @default.
• W2082319819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16053311" @default.
• W2082319819 hasPublicationYear "2005" @default.
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