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• W2082344469 abstract "V3 loop peptides from three different human immunodeficiency virus type 1 (HIV-1) strains were synthesized. BH10, ADA, and 89.6 strains whose infections are dependent on CXCR4, CCR5, and both, respectively, were selected. Co-transfection of luciferase reporter gene and corresponding envelope genes (HXB2, ADA, and 89.6) generate pseudotype viruses (HXB2/Luc, ADA/Luc, and 89.6/Luc). The effects of each peptide on the infection of U87 cells expressing CD4 and one of the coreceptors with all pseudotype viruses were evaluated. V3 loop peptide from BH10 (V3-BH10) alone increased the HXB2/Luc infection by 93% at 10μM. Both V3-ADA and V3-89.6 enhanced ADA/Luc infection by 38% and by 55% at 10μM, respectively. For 89.6/Luc infection, only V3-89.6 enhanced the infections on both target cells. V3-BH10 modulated the epitopes of coreceptor binding site and V2 loop of gp120 on HIV-1 IIIB infected H9 cells, indicating that V3 loop peptide activates viral gp120 and enhances infectivity." @default.
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• W2082344469 date "2002-09-01" @default.
• W2082344469 modified "2023-09-27" @default.
• W2082344469 title "Activation of gp120 of human immunodeficiency virus by their V3 loop-derived peptides" @default.
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• W2082344469 doi "https://doi.org/10.1016/s0006-291x(02)02253-2" @default.
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