Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082364100> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2082364100 endingPage "2080" @default.
- W2082364100 startingPage "2074" @default.
- W2082364100 abstract "The present manuscript describes development and validation of LC–MS/MS assay for the simultaneous quantitation of 97/78 and its active in-vivo metabolite 97/63 in monkey plasma using α-arteether as internal standard (IS). The method involves a single step protein precipitation using acetonitrile as extraction method. The analytes were separated on a Columbus C18 (50 mm × 2 mm i.d., 5 μm particle size) column by isocratic elution with acetonitrile:ammonium acetate buffer (pH 4, 10 mM) (80:20 v/v) at a flow rate of 0.45 mL/min, and analyzed by mass spectrometry in multiple reaction-monitoring (MRM) positive ion mode. The chromatographic run time was 4.0 min and the weighted (1/x2) calibration curves were linear over a range of 1.56–200 ng/mL. The method was linear for both the analytes with correlation coefficients >0.995. The intra-day and inter-day accuracy (% bias) and precisions (% RSD) of the assay were less than 6.27%. Both analytes were stable after three freeze-thaw cycles (% deviation <8.2) and also for 30 days in plasma (% deviation <6.7). The absolute recoveries of 97/78, 97/63 and internal standard (IS), from spiked plasma samples were >90%. The validated assay method, described here, was successfully applied to the pharmacokinetic study of 97/78 and its active in-vivo metabolite 97/63 in Rhesus monkeys." @default.
- W2082364100 created "2016-06-24" @default.
- W2082364100 creator A5035613509 @default.
- W2082364100 creator A5053656249 @default.
- W2082364100 creator A5065498407 @default.
- W2082364100 creator A5068258182 @default.
- W2082364100 creator A5080640567 @default.
- W2082364100 date "2009-07-01" @default.
- W2082364100 modified "2023-10-11" @default.
- W2082364100 title "Liquid chromatographic tandem mass spectrometric assay for quantification of 97/78 and its metabolite 97/63: A promising trioxane antimalarial in monkey plasma" @default.
- W2082364100 cites W1515542254 @default.
- W2082364100 cites W1871026043 @default.
- W2082364100 cites W1980121952 @default.
- W2082364100 cites W1981989068 @default.
- W2082364100 cites W1989671297 @default.
- W2082364100 cites W1999790407 @default.
- W2082364100 cites W2000718280 @default.
- W2082364100 cites W2040017007 @default.
- W2082364100 cites W2067051421 @default.
- W2082364100 cites W2072719341 @default.
- W2082364100 cites W2091210272 @default.
- W2082364100 cites W2175817553 @default.
- W2082364100 doi "https://doi.org/10.1016/j.jchromb.2009.05.044" @default.
- W2082364100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19520624" @default.
- W2082364100 hasPublicationYear "2009" @default.
- W2082364100 type Work @default.
- W2082364100 sameAs 2082364100 @default.
- W2082364100 citedByCount "12" @default.
- W2082364100 countsByYear W20823641002012 @default.
- W2082364100 countsByYear W20823641002013 @default.
- W2082364100 countsByYear W20823641002014 @default.
- W2082364100 countsByYear W20823641002021 @default.
- W2082364100 countsByYear W20823641002022 @default.
- W2082364100 crossrefType "journal-article" @default.
- W2082364100 hasAuthorship W2082364100A5035613509 @default.
- W2082364100 hasAuthorship W2082364100A5053656249 @default.
- W2082364100 hasAuthorship W2082364100A5065498407 @default.
- W2082364100 hasAuthorship W2082364100A5068258182 @default.
- W2082364100 hasAuthorship W2082364100A5080640567 @default.
- W2082364100 hasConcept C10390740 @default.
- W2082364100 hasConcept C119128265 @default.
- W2082364100 hasConcept C149151106 @default.
- W2082364100 hasConcept C162356407 @default.
- W2082364100 hasConcept C179998833 @default.
- W2082364100 hasConcept C185592680 @default.
- W2082364100 hasConcept C2777477808 @default.
- W2082364100 hasConcept C2780782300 @default.
- W2082364100 hasConcept C31827203 @default.
- W2082364100 hasConcept C43617362 @default.
- W2082364100 hasConcept C4725764 @default.
- W2082364100 hasConcept C55493867 @default.
- W2082364100 hasConcept C64489805 @default.
- W2082364100 hasConcept C71043704 @default.
- W2082364100 hasConcept C73826308 @default.
- W2082364100 hasConcept C74774607 @default.
- W2082364100 hasConceptScore W2082364100C10390740 @default.
- W2082364100 hasConceptScore W2082364100C119128265 @default.
- W2082364100 hasConceptScore W2082364100C149151106 @default.
- W2082364100 hasConceptScore W2082364100C162356407 @default.
- W2082364100 hasConceptScore W2082364100C179998833 @default.
- W2082364100 hasConceptScore W2082364100C185592680 @default.
- W2082364100 hasConceptScore W2082364100C2777477808 @default.
- W2082364100 hasConceptScore W2082364100C2780782300 @default.
- W2082364100 hasConceptScore W2082364100C31827203 @default.
- W2082364100 hasConceptScore W2082364100C43617362 @default.
- W2082364100 hasConceptScore W2082364100C4725764 @default.
- W2082364100 hasConceptScore W2082364100C55493867 @default.
- W2082364100 hasConceptScore W2082364100C64489805 @default.
- W2082364100 hasConceptScore W2082364100C71043704 @default.
- W2082364100 hasConceptScore W2082364100C73826308 @default.
- W2082364100 hasConceptScore W2082364100C74774607 @default.
- W2082364100 hasIssue "22" @default.
- W2082364100 hasLocation W20823641001 @default.
- W2082364100 hasOpenAccess W2082364100 @default.
- W2082364100 hasPrimaryLocation W20823641001 @default.
- W2082364100 hasRelatedWork W1998032538 @default.
- W2082364100 hasRelatedWork W2038454584 @default.
- W2082364100 hasRelatedWork W2077240333 @default.
- W2082364100 hasRelatedWork W2465213228 @default.
- W2082364100 hasRelatedWork W2767995033 @default.
- W2082364100 hasRelatedWork W2795477932 @default.
- W2082364100 hasRelatedWork W2973860202 @default.
- W2082364100 hasRelatedWork W2987815507 @default.
- W2082364100 hasRelatedWork W3087792627 @default.
- W2082364100 hasRelatedWork W4289523957 @default.
- W2082364100 hasVolume "877" @default.
- W2082364100 isParatext "false" @default.
- W2082364100 isRetracted "false" @default.
- W2082364100 magId "2082364100" @default.
- W2082364100 workType "article" @default.