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- W2082387377 abstract "ABSTRACT Most traditional biological assays are based on ensemble measurements on cells . Due to lack of the detection sensitivity and efficiency in sample preparation, anal yzing proteins in single cells have been challenging. Here we describe an assay-on-a-tip platform, and demonstrate an in situ, label-free technique to detect proteins inside single cells. Keywords: Single cells, surface plasmon resonance, optical fiber 1. INTRODUCTION Much of our knowledge in biology so far is based on ensemble measurements on cells 1 . However, the stochastic expression of genes, proteins and metabolites result in cellular heterogene ity. A major challenge is to understand how individua l cells process information and respond to stimuli. Traditional assays, such as enzyme-linked immunosorbent assay (ELISA) and western blot, lack the detection sensitivity and efficiency in sample preparation to achieve single cell capability . Emerging microfluidic techniques are constantly improving the detection and sample manipulation capability" @default.
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- W2082387377 date "2014-08-27" @default.
- W2082387377 modified "2023-09-28" @default.
- W2082387377 title "Nanoscale fiber tip probe for biomedical sensing" @default.
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- W2082387377 doi "https://doi.org/10.1117/12.2062485" @default.
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