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• W2082507324 abstract "An enzyme-linked immunosorbent assay-based displacement assay was developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Advantage was taken of the cross-reactivity of a monoclonal anti-2,4-D antibody toward 2-methyl-4-chlorophenoxyacetic acid (MCPA). MCPA was conjugated with bovine serum albumin (BSA), immobilized on the surface of a microtiter plate, and saturated with the anti-2,4-D antibody. Due to the low affinity of the antibody toward MCPA (cross-reactivity of approximately 30%), the addition of 2,4-D resulted in a displacement of the antibody. Remaining antibodies were subsequently detected using a peroxidase-labeled goat anti-mouse antibody. The detection limit was as low as 0.1 μg/liter for 2,4-D, which complies with the European Union Drinking Water Directives. When 2,4-D–BSA was used instead of MCPA–BSA conjugates, no significant displacement of bound antibody was observed." @default.
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• W2082507324 date "1997-10-01" @default.
• W2082507324 modified "2023-09-25" @default.
• W2082507324 title "Development of a Displacement Immunoassay by Exploiting Cross-Reactivity of a Monoclonal Antibody" @default.
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• W2082507324 doi "https://doi.org/10.1006/abio.1997.2301" @default.
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