Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082532569> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2082532569 endingPage "386" @default.
- W2082532569 startingPage "375" @default.
- W2082532569 abstract "A procedure is described for isolating amplified nucleoli from immature oocytes of Xenopus laevis. Adult ovaries are dissociated by digestion with collagenase-papain and immature, pigment-free oocytes (Dumont stages II and III) are isolated by a series of 1g sedimentation steps followed by centrifugation through 1.7 M sucrose. The immature oocytes are then homogenized and nucleoli are isolated by buoyant density banding in gradients of Metrizamide. Nucleoli isolated by this procedure have normal morphology by both light and electron microscopy. They contain intact ribosomal RNA precursor molecules and an active endogenous RNA polymerase which has the characteristics of the form I or nucleolar polymerase. The nucleoli also contain an enzymatic activity which relaxes superhelical turns in closed circular DNA. This activity follows RNA polymerase activity in a Metrizamide gradient suggesting that this enzyme may be involved in transcription. Electrophoresis of total nucleolar proteins on SDS-acrylamide gels shows most of the proteins migrating with protein from cytoplasmic ribosomes. Some nonribosomal proteins are clearly visible however, among which are proteins which comigrate with histones H2a, H2b, H3, and H4. Using this procedure nucleoli may be isolated completely free of any DNA component other than amplified ribosomal DNA. They represent the first case in which it has been possible to purify a single gene with its proteins still attached." @default.
- W2082532569 created "2016-06-24" @default.
- W2082532569 creator A5011800975 @default.
- W2082532569 creator A5033525419 @default.
- W2082532569 creator A5070297574 @default.
- W2082532569 date "1977-02-01" @default.
- W2082532569 modified "2023-10-02" @default.
- W2082532569 title "Isolation of ribosomal gene chromatin" @default.
- W2082532569 cites W1533065308 @default.
- W2082532569 cites W1590526003 @default.
- W2082532569 cites W1603290388 @default.
- W2082532569 cites W1964661746 @default.
- W2082532569 cites W1994193451 @default.
- W2082532569 cites W2012470401 @default.
- W2082532569 cites W2025202951 @default.
- W2082532569 cites W2053175555 @default.
- W2082532569 cites W2054109662 @default.
- W2082532569 cites W2064577776 @default.
- W2082532569 cites W2084609764 @default.
- W2082532569 cites W2089918024 @default.
- W2082532569 cites W2090262338 @default.
- W2082532569 cites W2095472025 @default.
- W2082532569 cites W2100837269 @default.
- W2082532569 cites W2104404316 @default.
- W2082532569 cites W2175826522 @default.
- W2082532569 doi "https://doi.org/10.1016/0012-1606(77)90180-4" @default.
- W2082532569 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/557006" @default.
- W2082532569 hasPublicationYear "1977" @default.
- W2082532569 type Work @default.
- W2082532569 sameAs 2082532569 @default.
- W2082532569 citedByCount "65" @default.
- W2082532569 countsByYear W20825325692013 @default.
- W2082532569 countsByYear W20825325692014 @default.
- W2082532569 countsByYear W20825325692018 @default.
- W2082532569 countsByYear W20825325692020 @default.
- W2082532569 crossrefType "journal-article" @default.
- W2082532569 hasAuthorship W2082532569A5011800975 @default.
- W2082532569 hasAuthorship W2082532569A5033525419 @default.
- W2082532569 hasAuthorship W2082532569A5070297574 @default.
- W2082532569 hasConcept C104317684 @default.
- W2082532569 hasConcept C153911025 @default.
- W2082532569 hasConcept C183873130 @default.
- W2082532569 hasConcept C190062978 @default.
- W2082532569 hasConcept C25281209 @default.
- W2082532569 hasConcept C2776449523 @default.
- W2082532569 hasConcept C552990157 @default.
- W2082532569 hasConcept C55493867 @default.
- W2082532569 hasConcept C67705224 @default.
- W2082532569 hasConcept C67905577 @default.
- W2082532569 hasConcept C82381507 @default.
- W2082532569 hasConcept C83640560 @default.
- W2082532569 hasConcept C86803240 @default.
- W2082532569 hasConcept C88478588 @default.
- W2082532569 hasConceptScore W2082532569C104317684 @default.
- W2082532569 hasConceptScore W2082532569C153911025 @default.
- W2082532569 hasConceptScore W2082532569C183873130 @default.
- W2082532569 hasConceptScore W2082532569C190062978 @default.
- W2082532569 hasConceptScore W2082532569C25281209 @default.
- W2082532569 hasConceptScore W2082532569C2776449523 @default.
- W2082532569 hasConceptScore W2082532569C552990157 @default.
- W2082532569 hasConceptScore W2082532569C55493867 @default.
- W2082532569 hasConceptScore W2082532569C67705224 @default.
- W2082532569 hasConceptScore W2082532569C67905577 @default.
- W2082532569 hasConceptScore W2082532569C82381507 @default.
- W2082532569 hasConceptScore W2082532569C83640560 @default.
- W2082532569 hasConceptScore W2082532569C86803240 @default.
- W2082532569 hasConceptScore W2082532569C88478588 @default.
- W2082532569 hasIssue "2" @default.
- W2082532569 hasLocation W20825325691 @default.
- W2082532569 hasLocation W20825325692 @default.
- W2082532569 hasOpenAccess W2082532569 @default.
- W2082532569 hasPrimaryLocation W20825325691 @default.
- W2082532569 hasRelatedWork W1545600460 @default.
- W2082532569 hasRelatedWork W1983370148 @default.
- W2082532569 hasRelatedWork W1997591085 @default.
- W2082532569 hasRelatedWork W2004180671 @default.
- W2082532569 hasRelatedWork W2032454324 @default.
- W2082532569 hasRelatedWork W2053793562 @default.
- W2082532569 hasRelatedWork W2081711908 @default.
- W2082532569 hasRelatedWork W2111720919 @default.
- W2082532569 hasRelatedWork W2143983551 @default.
- W2082532569 hasRelatedWork W2186954722 @default.
- W2082532569 hasVolume "55" @default.
- W2082532569 isParatext "false" @default.
- W2082532569 isRetracted "false" @default.
- W2082532569 magId "2082532569" @default.
- W2082532569 workType "article" @default.