FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082541318> ?p ?o ?g. }

• W2082541318 abstract "We were interested to read the recent publication by Vega et al. (1), which suggests that indole is an interspecies signal that causes Salmonella to become less susceptible to antibiotics due to activation of the oxidative stress response. However, we believe that there is an alternative explanation for their data: the drug tolerance phenotype is due to increased production of one or more multidrug efflux pumps. The drugs tested by Vega et al., ciprofloxacin and carbenicillin, are known substrates of the AcrB transporter, and indole has been shown to induce the production of efflux pumps in both Escherichia coli and Salmonella (2). In Salmonella, induction is mediated by increased expression of the transcriptional activator RamA, which regulates expression of acrAB (2). Therefore, the indole-induced drug tolerance seen by Vega et al. could result from induction of the multidrug resistance (MDR) AcrAB-TolC efflux system, leading to increased tolerance to these two drugs. This hypothesis is noted by the authors but dismissed after their RT-PCR experiments showed that expression of the ramA gene was reduced in the presence of indole. We are perplexed by this observation because it conflicts with all previous studies including measurements using gene reporter constructs, RT-PCR, microarray, and Western blotting (2), which reveal that ramA/RamA is induced by indole in numerous different Salmonella strains including strain LT2 used by Vega et al. in their experiments. Furthermore, the authors used these RT-PCR data as evidence that efflux is not involved in the phenotype. We believe that this is a mistaken assumption for two reasons. First, efflux was not measured. This is surprising because efflux of ciprofloxacin and other compounds can be measured easily using one of several published methods to quantify accumulation or efflux of fluorescent substrates (3, 4). Second, regulation of MDR efflux pumps in Gram-negative bacteria is complex and multifactorial. For instance, other transcription factors, including MarA, SoxS, and Rob, can also regulate expression of MDR efflux pumps, and indole induces expression of soxS in E. coli (5)." @default.
• W2082541318 created "2016-06-24" @default.
• W2082541318 creator A5001173964 @default.
• W2082541318 creator A5019987484 @default.
• W2082541318 creator A5082494281 @default.
• W2082541318 creator A5091502422 @default.
• W2082541318 date "2013-11-06" @default.
• W2082541318 modified "2023-09-27" @default.
• W2082541318 title "Alternative explanation for indole-induced antibiotic tolerance in <i>Salmonella</i>" @default.
• W2082541318 cites W128425680 @default.
• W2082541318 cites W1968258605 @default.
• W2082541318 cites W2012203449 @default.
• W2082541318 cites W2067566372 @default.
• W2082541318 cites W2118307652 @default.
• W2082541318 doi "https://doi.org/10.1073/pnas.1318318110" @default.
• W2082541318 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3845160" @default.
• W2082541318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24198338" @default.
• W2082541318 hasPublicationYear "2013" @default.
• W2082541318 type Work @default.
• W2082541318 sameAs 2082541318 @default.
• W2082541318 citedByCount "8" @default.
• W2082541318 countsByYear W20825413182013 @default.
• W2082541318 countsByYear W20825413182015 @default.
• W2082541318 countsByYear W20825413182019 @default.
• W2082541318 countsByYear W20825413182020 @default.
• W2082541318 countsByYear W20825413182021 @default.
• W2082541318 countsByYear W20825413182023 @default.
• W2082541318 crossrefType "journal-article" @default.
• W2082541318 hasAuthorship W2082541318A5001173964 @default.
• W2082541318 hasAuthorship W2082541318A5019987484 @default.
• W2082541318 hasAuthorship W2082541318A5082494281 @default.
• W2082541318 hasAuthorship W2082541318A5091502422 @default.
• W2082541318 hasBestOaLocation W20825413181 @default.
• W2082541318 hasConcept C185592680 @default.
• W2082541318 hasConcept C192527728 @default.
• W2082541318 hasConcept C2781065037 @default.
• W2082541318 hasConcept C501593827 @default.
• W2082541318 hasConcept C523546767 @default.
• W2082541318 hasConcept C54355233 @default.
• W2082541318 hasConcept C71240020 @default.
• W2082541318 hasConcept C86803240 @default.
• W2082541318 hasConcept C89423630 @default.
• W2082541318 hasConceptScore W2082541318C185592680 @default.
• W2082541318 hasConceptScore W2082541318C192527728 @default.
• W2082541318 hasConceptScore W2082541318C2781065037 @default.
• W2082541318 hasConceptScore W2082541318C501593827 @default.
• W2082541318 hasConceptScore W2082541318C523546767 @default.
• W2082541318 hasConceptScore W2082541318C54355233 @default.
• W2082541318 hasConceptScore W2082541318C71240020 @default.
• W2082541318 hasConceptScore W2082541318C86803240 @default.
• W2082541318 hasConceptScore W2082541318C89423630 @default.
• W2082541318 hasIssue "48" @default.
• W2082541318 hasLocation W20825413181 @default.
• W2082541318 hasLocation W20825413182 @default.
• W2082541318 hasLocation W20825413183 @default.
• W2082541318 hasLocation W20825413184 @default.
• W2082541318 hasLocation W20825413185 @default.
• W2082541318 hasOpenAccess W2082541318 @default.
• W2082541318 hasPrimaryLocation W20825413181 @default.
• W2082541318 hasRelatedWork W1987118295 @default.
• W2082541318 hasRelatedWork W1994655645 @default.
• W2082541318 hasRelatedWork W2073779326 @default.
• W2082541318 hasRelatedWork W2335684962 @default.
• W2082541318 hasRelatedWork W2346073318 @default.
• W2082541318 hasRelatedWork W2784164631 @default.
• W2082541318 hasRelatedWork W2950408570 @default.
• W2082541318 hasRelatedWork W2951340964 @default.
• W2082541318 hasRelatedWork W2952360933 @default.
• W2082541318 hasRelatedWork W4312871856 @default.
• W2082541318 hasVolume "110" @default.
• W2082541318 isParatext "false" @default.
• W2082541318 isRetracted "false" @default.
• W2082541318 magId "2082541318" @default.
• W2082541318 workType "article" @default.