FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082572007> ?p ?o ?g. }

• W2082572007 endingPage "134" @default.
• W2082572007 startingPage "128" @default.
• W2082572007 abstract "We have compared the effectiveness of culture-based methods and a DNA-based method for the detection, of Vibrio vulnificus from seawater and three types of shellfish collected from the coastal waters of Valencia, Spain. For culture-based method, we used two selective media, thiosulphate-citrate-bile salts-sucrose (TCBS), and cellobiose-polymyxin B-colistin (CPC) agars with and without previous enrichment in alkaline-saline-peptone-water (APWS). Presumptive colonies were confirmed as V. vulnificus by the polymerase chain reaction (PCR) using previously described 23S rRNA V. vulficus-specific sequences as primers (Dvu 9V and Dvu 45R). Direct detection was accomplished by a nested-PCR procedure developed for environmental samples, with the above mentioned primers for the second amplificaton. Of 32 seawater samples, only one yielded positive results by direct detection by PCR, whereas five were positive by culture methods. Of the 32 bivalve samples, two were positive by PCR and five by culture methods. From a total of 675 presumptive colonies selected on the two media, only 48 (20 from seawater and 28 from bivalves) were confirmed as V. vulnificus by PCR. Forty-six V. vulnificus isolates were obtained after enrichment and only two after direct inoculation on CPC. Except for one sampling, positive results by direct detection did not correlate with confirmed strains obtained from culture media. API 20E profiles were recorded for all isolates previously identified as V. vulnificus, revealing that around 20% of the strains were sucrose-positive. For our samples, the best strategy consisted in the combination of culture based methods (3 h enrichment in APWS at 40 °C followed by CPC at the same temperature) and DNA-based procedures (specific PCR amplification of the presumptive colonies with primers Dvu 9V and Dvu 45R), which allowed the detection and accurate identification of V. vulnificus in less than 48h. This is the first report on the detection of cells of V. vulnificus naturally present in seawater and edible shellfish in the Spanish Mediterranean coast." @default.
• W2082572007 created "2016-06-24" @default.
• W2082572007 creator A5009995705 @default.
• W2082572007 creator A5012537431 @default.
• W2082572007 creator A5012724891 @default.
• W2082572007 creator A5034121124 @default.
• W2082572007 date "1998-03-01" @default.
• W2082572007 modified "2023-10-14" @default.
• W2082572007 title "A Comparison of Strategies for the Detection and Recovery of Vibrio vulnificus from Marine Samples of the Western Mediterranean coast" @default.
• W2082572007 cites W1530772074 @default.
• W2082572007 cites W1548957515 @default.
• W2082572007 cites W1561569036 @default.
• W2082572007 cites W1588136179 @default.
• W2082572007 cites W1776583453 @default.
• W2082572007 cites W1843363152 @default.
• W2082572007 cites W189156639 @default.
• W2082572007 cites W1937235222 @default.
• W2082572007 cites W1948614359 @default.
• W2082572007 cites W1966762157 @default.
• W2082572007 cites W1986296689 @default.
• W2082572007 cites W1989745944 @default.
• W2082572007 cites W1991281549 @default.
• W2082572007 cites W1994684228 @default.
• W2082572007 cites W2001097267 @default.
• W2082572007 cites W2023704653 @default.
• W2082572007 cites W2048548850 @default.
• W2082572007 cites W2077114502 @default.
• W2082572007 cites W2095077686 @default.
• W2082572007 cites W2102639664 @default.
• W2082572007 cites W2112669137 @default.
• W2082572007 cites W2114429356 @default.
• W2082572007 cites W2119790048 @default.
• W2082572007 cites W2130849933 @default.
• W2082572007 cites W2157415604 @default.
• W2082572007 cites W2157915435 @default.
• W2082572007 cites W2167678721 @default.
• W2082572007 doi "https://doi.org/10.1016/s0723-2020(98)80016-7" @default.
• W2082572007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9741117" @default.
• W2082572007 hasPublicationYear "1998" @default.
• W2082572007 type Work @default.
• W2082572007 sameAs 2082572007 @default.
• W2082572007 citedByCount "23" @default.
• W2082572007 countsByYear W20825720072013 @default.
• W2082572007 countsByYear W20825720072014 @default.
• W2082572007 countsByYear W20825720072015 @default.
• W2082572007 countsByYear W20825720072022 @default.
• W2082572007 crossrefType "journal-article" @default.
• W2082572007 hasAuthorship W2082572007A5009995705 @default.
• W2082572007 hasAuthorship W2082572007A5012537431 @default.
• W2082572007 hasAuthorship W2082572007A5012724891 @default.
• W2082572007 hasAuthorship W2082572007A5034121124 @default.
• W2082572007 hasConcept C104317684 @default.
• W2082572007 hasConcept C153613900 @default.
• W2082572007 hasConcept C158278297 @default.
• W2082572007 hasConcept C18903297 @default.
• W2082572007 hasConcept C197248824 @default.
• W2082572007 hasConcept C2776364969 @default.
• W2082572007 hasConcept C2776422119 @default.
• W2082572007 hasConcept C2778458500 @default.
• W2082572007 hasConcept C2778660310 @default.
• W2082572007 hasConcept C2781028344 @default.
• W2082572007 hasConcept C2909208804 @default.
• W2082572007 hasConcept C49105822 @default.
• W2082572007 hasConcept C505870484 @default.
• W2082572007 hasConcept C523546767 @default.
• W2082572007 hasConcept C54355233 @default.
• W2082572007 hasConcept C55493867 @default.
• W2082572007 hasConcept C86803240 @default.
• W2082572007 hasConcept C89423630 @default.
• W2082572007 hasConceptScore W2082572007C104317684 @default.
• W2082572007 hasConceptScore W2082572007C153613900 @default.
• W2082572007 hasConceptScore W2082572007C158278297 @default.
• W2082572007 hasConceptScore W2082572007C18903297 @default.
• W2082572007 hasConceptScore W2082572007C197248824 @default.
• W2082572007 hasConceptScore W2082572007C2776364969 @default.
• W2082572007 hasConceptScore W2082572007C2776422119 @default.
• W2082572007 hasConceptScore W2082572007C2778458500 @default.
• W2082572007 hasConceptScore W2082572007C2778660310 @default.
• W2082572007 hasConceptScore W2082572007C2781028344 @default.
• W2082572007 hasConceptScore W2082572007C2909208804 @default.
• W2082572007 hasConceptScore W2082572007C49105822 @default.
• W2082572007 hasConceptScore W2082572007C505870484 @default.
• W2082572007 hasConceptScore W2082572007C523546767 @default.
• W2082572007 hasConceptScore W2082572007C54355233 @default.
• W2082572007 hasConceptScore W2082572007C55493867 @default.
• W2082572007 hasConceptScore W2082572007C86803240 @default.
• W2082572007 hasConceptScore W2082572007C89423630 @default.
• W2082572007 hasIssue "1" @default.
• W2082572007 hasLocation W20825720071 @default.
• W2082572007 hasLocation W20825720072 @default.
• W2082572007 hasOpenAccess W2082572007 @default.
• W2082572007 hasPrimaryLocation W20825720071 @default.
• W2082572007 hasRelatedWork W1529235499 @default.
• W2082572007 hasRelatedWork W1529433353 @default.
• W2082572007 hasRelatedWork W1948614359 @default.
• W2082572007 hasRelatedWork W2010955096 @default.
• W2082572007 hasRelatedWork W2121081323 @default.
• W2082572007 hasRelatedWork W2124181705 @default.

• next