Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082600894> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2082600894 endingPage "1723" @default.
- W2082600894 startingPage "1714" @default.
- W2082600894 abstract "beta-Glucosidase is necessary for the bioconversion of glycosidic phytochemicals in food. Two Bifidobacterium strains (Bifidobacterium animalis subsp. lactis SH5 and B. animalis subsp. lactis RD68) with relatively high beta- glucosidase activities were selected among 46 lactic acid bacteria. A beta-glucosidase gene (bbg572) from B. lactis was shotgun cloned, fully sequenced, and analyzed for its transcription start site, structural gene, and deduced transcriptional terminator. The structural gene of bbg572 was 1,383 bp. Based on amino sequence similarities, bbg572 was assigned to family 1 of the glycosyl hydrolases. To overexpress bbg572 in Bifidobacterium, several bifidobacteria expression vectors were constructed by combining several promoters and a terminator sequence from different bifidobacteria. The maximum activity of recombinant Bbg572 was achieved when it was expressed under its own promoter and terminator. Its enzyme activity increased 31-fold compared with those of its parental strains. The optimal pH for Bbg572 was pH 6.0. Bbg572 was stable at 37-40 degrees C. It hydrolyzed isoflavones, quercetins, and disaccharides with various beta-glucoside linkages. Bbg572 also converted the ginsenosides Rb1 and Rb2. These results suggest that this new beta-glucosidase-positive Bifidobacterium transformant can be utilized for the production of specific aglycone products." @default.
- W2082600894 created "2016-06-24" @default.
- W2082600894 creator A5036601136 @default.
- W2082600894 creator A5037703743 @default.
- W2082600894 creator A5042272173 @default.
- W2082600894 date "2012-12-28" @default.
- W2082600894 modified "2023-10-09" @default.
- W2082600894 title "Identification of the β-Glucosidase Gene from Bifidobacterium animalis subsp. lactis and Its Expression in B. bifidum BGN4" @default.
- W2082600894 doi "https://doi.org/10.4014/jmb.1208.08028" @default.
- W2082600894 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23221535" @default.
- W2082600894 hasPublicationYear "2012" @default.
- W2082600894 type Work @default.
- W2082600894 sameAs 2082600894 @default.
- W2082600894 citedByCount "22" @default.
- W2082600894 countsByYear W20826008942013 @default.
- W2082600894 countsByYear W20826008942014 @default.
- W2082600894 countsByYear W20826008942015 @default.
- W2082600894 countsByYear W20826008942016 @default.
- W2082600894 countsByYear W20826008942017 @default.
- W2082600894 countsByYear W20826008942018 @default.
- W2082600894 countsByYear W20826008942019 @default.
- W2082600894 countsByYear W20826008942020 @default.
- W2082600894 countsByYear W20826008942021 @default.
- W2082600894 countsByYear W20826008942022 @default.
- W2082600894 crossrefType "journal-article" @default.
- W2082600894 hasAuthorship W2082600894A5036601136 @default.
- W2082600894 hasAuthorship W2082600894A5037703743 @default.
- W2082600894 hasAuthorship W2082600894A5042272173 @default.
- W2082600894 hasConcept C100544194 @default.
- W2082600894 hasConcept C104317684 @default.
- W2082600894 hasConcept C116403925 @default.
- W2082600894 hasConcept C121332964 @default.
- W2082600894 hasConcept C1276947 @default.
- W2082600894 hasConcept C141603559 @default.
- W2082600894 hasConcept C178046730 @default.
- W2082600894 hasConcept C181199279 @default.
- W2082600894 hasConcept C2777945686 @default.
- W2082600894 hasConcept C2778641530 @default.
- W2082600894 hasConcept C2780206646 @default.
- W2082600894 hasConcept C2780305828 @default.
- W2082600894 hasConcept C2780412358 @default.
- W2082600894 hasConcept C55493867 @default.
- W2082600894 hasConcept C86803240 @default.
- W2082600894 hasConceptScore W2082600894C100544194 @default.
- W2082600894 hasConceptScore W2082600894C104317684 @default.
- W2082600894 hasConceptScore W2082600894C116403925 @default.
- W2082600894 hasConceptScore W2082600894C121332964 @default.
- W2082600894 hasConceptScore W2082600894C1276947 @default.
- W2082600894 hasConceptScore W2082600894C141603559 @default.
- W2082600894 hasConceptScore W2082600894C178046730 @default.
- W2082600894 hasConceptScore W2082600894C181199279 @default.
- W2082600894 hasConceptScore W2082600894C2777945686 @default.
- W2082600894 hasConceptScore W2082600894C2778641530 @default.
- W2082600894 hasConceptScore W2082600894C2780206646 @default.
- W2082600894 hasConceptScore W2082600894C2780305828 @default.
- W2082600894 hasConceptScore W2082600894C2780412358 @default.
- W2082600894 hasConceptScore W2082600894C55493867 @default.
- W2082600894 hasConceptScore W2082600894C86803240 @default.
- W2082600894 hasIssue "12" @default.
- W2082600894 hasLocation W20826008941 @default.
- W2082600894 hasLocation W20826008942 @default.
- W2082600894 hasOpenAccess W2082600894 @default.
- W2082600894 hasPrimaryLocation W20826008941 @default.
- W2082600894 hasRelatedWork W1036907692 @default.
- W2082600894 hasRelatedWork W2011045516 @default.
- W2082600894 hasRelatedWork W2022715414 @default.
- W2082600894 hasRelatedWork W2036057672 @default.
- W2082600894 hasRelatedWork W2044271159 @default.
- W2082600894 hasRelatedWork W2045664599 @default.
- W2082600894 hasRelatedWork W2048887584 @default.
- W2082600894 hasRelatedWork W2082600894 @default.
- W2082600894 hasRelatedWork W2270442868 @default.
- W2082600894 hasRelatedWork W3115498783 @default.
- W2082600894 hasVolume "22" @default.
- W2082600894 isParatext "false" @default.
- W2082600894 isRetracted "false" @default.
- W2082600894 magId "2082600894" @default.
- W2082600894 workType "article" @default.