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• W2082642009 abstract "The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species observed on electrophoresis are believed to be a circular monomeric form, a linear form derived from it, a circular dimeric form, and the corresponding linear dimer. Two DNA components were demonstrated by sedimentation velocity in the analytical ultracentrifuge and by zone sedimentation in sucrose gradients. These corresponded to a circular monomeric form and a circular dimeric form; no evidence for supercoiled forms was found by sedimentation in either neutral or denaturing solvents. Bacteriophage 643 DNA was sensitive to cleavage by single-strand-specific nuclease S1 and could be labeled in vitro by the PolI-catalyzed incorporation of [alpha-32P]dATP into the molecule under conditions that did not permit nick translation, suggesting that the circular duplex molecule is interrupted by a single-stranded gap." @default.
• W2082642009 created "2016-06-24" @default.
• W2082642009 creator A5022181398 @default.
• W2082642009 creator A5032178182 @default.
• W2082642009 date "1984-03-01" @default.
• W2082642009 modified "2023-09-24" @default.
• W2082642009 title "The DNA of bacteriphage 643: Isolation and properties of the DNA of a bacteriphage infecting lactic streptococci" @default.
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• W2082642009 doi "https://doi.org/10.1016/0042-6822(84)90406-9" @default.
• W2082642009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6324460" @default.
• W2082642009 hasPublicationYear "1984" @default.
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