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- W2082645878 abstract "By means of Ca2+- and K+-selective microelectrodes, the changes in intracellular free Ca2+ and K+ were measured during the hyperpolarizing responses induced by ATP, complement and β-lipoprotein in mouse fibroblastic L cells. The cytoplasmic Ca2+ concentration ([Ca]i) was about 0.4 μM in the resting state. The hyperpolarizing responses always coincided with a phasic increase in [Ca]i. ATP or β-lipoprotein induced about a 2-fold rise in [Ca]i, and complement did up to 3-fold. Both the hyperpolarizing responses and [Ca]i increases were prevented by removal of external Ca2+ or by application of a Ca-channel blocker, nifedipine. Quinine, a Ca-activated K-channel inhibitor, suppressed the hyperpolarizing responses but not the [Ca]i increases. During the hyperpolarizing response, the intracellular free K+ concentration gradually decreased from about 120 to 110 mM. Thus, it is concluded that ATP, complement and β-lipoprotein caused a transient elevation of cytoplasmic free Ca2+ due to Ca2+ influxes, thereby inducing electrical membrane responses through activation of Ca-dependent K-channels in the fibroblasts." @default.
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- W2082645878 title "Increases in cytosolic free Ca2+ induced by ATP, complement and β-lipoprotein in mouse L fibroblasts" @default.
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- W2082645878 doi "https://doi.org/10.1016/0006-291x(85)91021-6" @default.
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