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- W2082649573 abstract "The transcript accumulation for subunits 2–6 of photosystem I (PSI) was determined after irradiation of 5.5 days old etiolated seedlings of Spinacea oleracea. Homologous gene probes were used for Northern blot and slot blot analysis. For comparison, heterologous probes of a light-regulated (cab) and a constitutively expressed (actin) gene were included. In dark-grown seedlings, only the PSI-4 transcript was detectable in relatively high amounts apart from the actin transcript; all other transcripts were present only in trace amounts. The kinetic of transcript accumulation was nearly linear during 8 h of continuous irradiation with white light; the increase for PSI-2, −3, −5, −6 and cab transcripts was 7–10-fold within this time. The level of the PSI-4 transcript increased only 2.5-fold in the same experiments. After short (1–5 min) pulses of red light, a lag phase of about 1 h was found for accumulation of all light-regulated transcripts except for accumulation of PSI-4 which occurred immediately without lag phase. The maximum level for PSI-2, −3, −5, −6 and cab transcripts was reached at about 5–6 h after the red light pulse. A far-red light pulse given immediately after the red light pulse decreased the transcript levels to those found after a far-red light pulse alone. We conclude that transcription of the genes coding for PSI subunits 2–6 is regulated by phytochrome." @default.
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- W2082649573 date "1991-11-01" @default.
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- W2082649573 title "Phytochrome-dependent mRNA accumulation for nuclear coded photosystem I subunits in spinach seedlings" @default.
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- W2082649573 doi "https://doi.org/10.1016/1011-1344(91)80255-g" @default.
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