FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082653146> ?p ?o ?g. }

• W2082653146 endingPage "141" @default.
• W2082653146 startingPage "136" @default.
• W2082653146 abstract "Purpose Adenoviral vector-based gene therapy is a promising technique for the delivery of growth factors to tendons. The objective of this study was to determine whether rabbit flexor tendons could be transduced effectively by adenoviral vectors and whether the introduction of adenoviral vectors would cause a notable local inflammatory response. Methods Recombinant adenoviruses expressing green fluorescent protein (AdGFP) or BMP-13 (AdBMP-13) were constructed and 3 different viral titers (1 x 107, 1 x 108, and 1 x 109) were tested in this study. The second through fifth tendons of the forepaws and hindpaws of a New Zealand white rabbit were identified surgically and injected with different viral titers of adenoviruses. The fifth tendon was used as a control. The tendons were harvested 12 days after surgery. The retrieved tendons were sectioned to measure transgene expression, as well as for histologic evaluation. Results At all tested viral titers an efficient dose-dependent transgene expression was detected in all samples at 12 days after injection. At the highest dose the injection sites were notable for lymphocytic infiltration, suggesting that injected adenoviral vectors can illicit some local inflammatory response. Lymphocytic infiltration was much less apparent, however, in the tendons injected with lower titers of adenoviral vectors. There was no evidence of a massive inflammatory response and/or cell death. Conclusions Our findings show that adenovirus-based gene therapy is an efficient means of gene delivery to rabbit flexor tendons. Transduction efficiency of transgenes was dose dependent across the tested titers, although adenovirus-induced inflammation was notable only at the highest titer. This indicates that efficient gene transfer without notable local inflammatory response may be achieved by using the lower titers. Although adenovirus-induced inflammation can be minimized by using lower viral titers, its impact on adhesion formation in the long term remains unknown. Adenoviral vector-based gene therapy is a promising technique for the delivery of growth factors to tendons. The objective of this study was to determine whether rabbit flexor tendons could be transduced effectively by adenoviral vectors and whether the introduction of adenoviral vectors would cause a notable local inflammatory response. Recombinant adenoviruses expressing green fluorescent protein (AdGFP) or BMP-13 (AdBMP-13) were constructed and 3 different viral titers (1 x 107, 1 x 108, and 1 x 109) were tested in this study. The second through fifth tendons of the forepaws and hindpaws of a New Zealand white rabbit were identified surgically and injected with different viral titers of adenoviruses. The fifth tendon was used as a control. The tendons were harvested 12 days after surgery. The retrieved tendons were sectioned to measure transgene expression, as well as for histologic evaluation. At all tested viral titers an efficient dose-dependent transgene expression was detected in all samples at 12 days after injection. At the highest dose the injection sites were notable for lymphocytic infiltration, suggesting that injected adenoviral vectors can illicit some local inflammatory response. Lymphocytic infiltration was much less apparent, however, in the tendons injected with lower titers of adenoviral vectors. There was no evidence of a massive inflammatory response and/or cell death. Our findings show that adenovirus-based gene therapy is an efficient means of gene delivery to rabbit flexor tendons. Transduction efficiency of transgenes was dose dependent across the tested titers, although adenovirus-induced inflammation was notable only at the highest titer. This indicates that efficient gene transfer without notable local inflammatory response may be achieved by using the lower titers. Although adenovirus-induced inflammation can be minimized by using lower viral titers, its impact on adhesion formation in the long term remains unknown." @default.
• W2082653146 created "2016-06-24" @default.
• W2082653146 creator A5036329712 @default.
• W2082653146 creator A5044275494 @default.
• W2082653146 creator A5070578608 @default.
• W2082653146 creator A5079286604 @default.
• W2082653146 creator A5081240561 @default.
• W2082653146 creator A5081499428 @default.
• W2082653146 date "2005-01-01" @default.
• W2082653146 modified "2023-09-25" @default.
• W2082653146 title "Characterization of adenovirus-mediated gene transfer in rabbit flexor tendons" @default.
• W2082653146 cites W1875759935 @default.
• W2082653146 cites W1937780100 @default.
• W2082653146 cites W1963554792 @default.
• W2082653146 cites W1972880992 @default.
• W2082653146 cites W1977468422 @default.
• W2082653146 cites W1986831882 @default.
• W2082653146 cites W1986917953 @default.
• W2082653146 cites W1996309150 @default.
• W2082653146 cites W2000268243 @default.
• W2082653146 cites W2003955696 @default.
• W2082653146 cites W2005568210 @default.
• W2082653146 cites W2017614842 @default.
• W2082653146 cites W2032667301 @default.
• W2082653146 cites W2052612816 @default.
• W2082653146 cites W2079411521 @default.
• W2082653146 cites W2122532291 @default.
• W2082653146 cites W2125532158 @default.
• W2082653146 cites W2464378433 @default.
• W2082653146 cites W26626228 @default.
• W2082653146 cites W4253422011 @default.
• W2082653146 doi "https://doi.org/10.1016/j.jhsa.2004.08.010" @default.
• W2082653146 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15680569" @default.
• W2082653146 hasPublicationYear "2005" @default.
• W2082653146 type Work @default.
• W2082653146 sameAs 2082653146 @default.
• W2082653146 citedByCount "35" @default.
• W2082653146 countsByYear W20826531462012 @default.
• W2082653146 countsByYear W20826531462013 @default.
• W2082653146 countsByYear W20826531462014 @default.
• W2082653146 countsByYear W20826531462016 @default.
• W2082653146 countsByYear W20826531462017 @default.
• W2082653146 countsByYear W20826531462018 @default.
• W2082653146 countsByYear W20826531462020 @default.
• W2082653146 countsByYear W20826531462021 @default.
• W2082653146 countsByYear W20826531462022 @default.
• W2082653146 crossrefType "journal-article" @default.
• W2082653146 hasAuthorship W2082653146A5036329712 @default.
• W2082653146 hasAuthorship W2082653146A5044275494 @default.
• W2082653146 hasAuthorship W2082653146A5070578608 @default.
• W2082653146 hasAuthorship W2082653146A5079286604 @default.
• W2082653146 hasAuthorship W2082653146A5081240561 @default.
• W2082653146 hasAuthorship W2082653146A5081499428 @default.
• W2082653146 hasConcept C102230213 @default.
• W2082653146 hasConcept C104317684 @default.
• W2082653146 hasConcept C111599444 @default.
• W2082653146 hasConcept C135983454 @default.
• W2082653146 hasConcept C142724271 @default.
• W2082653146 hasConcept C15152581 @default.
• W2082653146 hasConcept C153911025 @default.
• W2082653146 hasConcept C159047783 @default.
• W2082653146 hasConcept C2522874641 @default.
• W2082653146 hasConcept C2776954459 @default.
• W2082653146 hasConcept C32470452 @default.
• W2082653146 hasConcept C32611913 @default.
• W2082653146 hasConcept C40767141 @default.
• W2082653146 hasConcept C55493867 @default.
• W2082653146 hasConcept C71924100 @default.
• W2082653146 hasConcept C86803240 @default.
• W2082653146 hasConceptScore W2082653146C102230213 @default.
• W2082653146 hasConceptScore W2082653146C104317684 @default.
• W2082653146 hasConceptScore W2082653146C111599444 @default.
• W2082653146 hasConceptScore W2082653146C135983454 @default.
• W2082653146 hasConceptScore W2082653146C142724271 @default.
• W2082653146 hasConceptScore W2082653146C15152581 @default.
• W2082653146 hasConceptScore W2082653146C153911025 @default.
• W2082653146 hasConceptScore W2082653146C159047783 @default.
• W2082653146 hasConceptScore W2082653146C2522874641 @default.
• W2082653146 hasConceptScore W2082653146C2776954459 @default.
• W2082653146 hasConceptScore W2082653146C32470452 @default.
• W2082653146 hasConceptScore W2082653146C32611913 @default.
• W2082653146 hasConceptScore W2082653146C40767141 @default.
• W2082653146 hasConceptScore W2082653146C55493867 @default.
• W2082653146 hasConceptScore W2082653146C71924100 @default.
• W2082653146 hasConceptScore W2082653146C86803240 @default.
• W2082653146 hasIssue "1" @default.
• W2082653146 hasLocation W20826531461 @default.
• W2082653146 hasLocation W20826531462 @default.
• W2082653146 hasOpenAccess W2082653146 @default.
• W2082653146 hasPrimaryLocation W20826531461 @default.
• W2082653146 hasRelatedWork W1981864943 @default.
• W2082653146 hasRelatedWork W1986851889 @default.
• W2082653146 hasRelatedWork W1994284515 @default.
• W2082653146 hasRelatedWork W1998100163 @default.
• W2082653146 hasRelatedWork W2001489295 @default.
• W2082653146 hasRelatedWork W204748332 @default.
• W2082653146 hasRelatedWork W2062787494 @default.
• W2082653146 hasRelatedWork W2090270527 @default.

• next