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• W2082765152 abstract "Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis." @default.
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• W2082765152 date "2009-03-01" @default.
• W2082765152 modified "2023-10-18" @default.
• W2082765152 title "Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens" @default.
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• W2082765152 doi "https://doi.org/10.1007/s11427-009-0008-5" @default.
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