Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082783706> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2082783706 endingPage "31" @default.
- W2082783706 startingPage "27" @default.
- W2082783706 abstract "The study of the changes in the steady-state levels of the early trypsin (ET) messenger RNA (mRNA) was used as a sensitive assay for measuring the effects of recombinant juvenile hormone esterase (rJHE) on juvenile hormone (JH)-dependent gene expression in mosquitoes. ET is a female-specific protease present in the midgut of the yellow fever mosquito Aedes aegypti during the first few hours after ingestion of a blood meal. Transcription of the early trypsin gene is part of the normal postemergence maturation of the midgut in the adult female, and it is regulated by JH. JHE was cloned from Heliothis virescens and expressed in a baculovirus vector. Injection of rJHE into mosquitoes resulted in an increase of JHE activity in the haemolymph. Injection of rJHE into newly emerged adult females delayed the normal increase in steady-state levels of ET mRNA observed in controls. Topically applied methoprene (a JH analogue) reversed the effect of rJHE. Injection of increasing concentrations of rJHE into 3-day-old unfed females resulted in a dose-dependent decrease in the steady-state levels of ET mRNA after 24 h. The effect of rJHE was transient, once the enzyme was cleared (72 h after injection), the steady-state levels of ET mRNA were restored. The injection of rJHE is an effective tool for modifying JH-dependent expression of the early trypsin gene in mosquitoes." @default.
- W2082783706 created "2016-06-24" @default.
- W2082783706 creator A5038260469 @default.
- W2082783706 creator A5038969156 @default.
- W2082783706 creator A5085742825 @default.
- W2082783706 creator A5090162849 @default.
- W2082783706 date "2000-02-01" @default.
- W2082783706 modified "2023-10-16" @default.
- W2082783706 title "Recombinant juvenile hormone esterase, an effective tool for modifying juvenile hormone-dependent expression of the early trypsin gene in mosquitoes" @default.
- W2082783706 cites W1970138152 @default.
- W2082783706 cites W1972844836 @default.
- W2082783706 cites W1984121095 @default.
- W2082783706 cites W2022242881 @default.
- W2082783706 cites W2036167482 @default.
- W2082783706 cites W2043663225 @default.
- W2082783706 cites W2074554676 @default.
- W2082783706 cites W2075350439 @default.
- W2082783706 cites W2082418162 @default.
- W2082783706 cites W2154188669 @default.
- W2082783706 cites W2159567428 @default.
- W2082783706 cites W2171528309 @default.
- W2082783706 cites W39448942 @default.
- W2082783706 cites W4231219026 @default.
- W2082783706 cites W4246050176 @default.
- W2082783706 doi "https://doi.org/10.1046/j.1365-2583.2000.00154.x" @default.
- W2082783706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10672068" @default.
- W2082783706 hasPublicationYear "2000" @default.
- W2082783706 type Work @default.
- W2082783706 sameAs 2082783706 @default.
- W2082783706 citedByCount "13" @default.
- W2082783706 countsByYear W20827837062013 @default.
- W2082783706 countsByYear W20827837062017 @default.
- W2082783706 countsByYear W20827837062018 @default.
- W2082783706 countsByYear W20827837062019 @default.
- W2082783706 countsByYear W20827837062020 @default.
- W2082783706 crossrefType "journal-article" @default.
- W2082783706 hasAuthorship W2082783706A5038260469 @default.
- W2082783706 hasAuthorship W2082783706A5038969156 @default.
- W2082783706 hasAuthorship W2082783706A5085742825 @default.
- W2082783706 hasAuthorship W2082783706A5090162849 @default.
- W2082783706 hasConcept C100068826 @default.
- W2082783706 hasConcept C104317684 @default.
- W2082783706 hasConcept C105580179 @default.
- W2082783706 hasConcept C126322002 @default.
- W2082783706 hasConcept C134018914 @default.
- W2082783706 hasConcept C150149183 @default.
- W2082783706 hasConcept C150194340 @default.
- W2082783706 hasConcept C153911025 @default.
- W2082783706 hasConcept C173758957 @default.
- W2082783706 hasConcept C181199279 @default.
- W2082783706 hasConcept C2777775583 @default.
- W2082783706 hasConcept C2780340462 @default.
- W2082783706 hasConcept C55493867 @default.
- W2082783706 hasConcept C59822182 @default.
- W2082783706 hasConcept C71220013 @default.
- W2082783706 hasConcept C71315377 @default.
- W2082783706 hasConcept C71924100 @default.
- W2082783706 hasConcept C86803240 @default.
- W2082783706 hasConceptScore W2082783706C100068826 @default.
- W2082783706 hasConceptScore W2082783706C104317684 @default.
- W2082783706 hasConceptScore W2082783706C105580179 @default.
- W2082783706 hasConceptScore W2082783706C126322002 @default.
- W2082783706 hasConceptScore W2082783706C134018914 @default.
- W2082783706 hasConceptScore W2082783706C150149183 @default.
- W2082783706 hasConceptScore W2082783706C150194340 @default.
- W2082783706 hasConceptScore W2082783706C153911025 @default.
- W2082783706 hasConceptScore W2082783706C173758957 @default.
- W2082783706 hasConceptScore W2082783706C181199279 @default.
- W2082783706 hasConceptScore W2082783706C2777775583 @default.
- W2082783706 hasConceptScore W2082783706C2780340462 @default.
- W2082783706 hasConceptScore W2082783706C55493867 @default.
- W2082783706 hasConceptScore W2082783706C59822182 @default.
- W2082783706 hasConceptScore W2082783706C71220013 @default.
- W2082783706 hasConceptScore W2082783706C71315377 @default.
- W2082783706 hasConceptScore W2082783706C71924100 @default.
- W2082783706 hasConceptScore W2082783706C86803240 @default.
- W2082783706 hasIssue "1" @default.
- W2082783706 hasLocation W20827837061 @default.
- W2082783706 hasLocation W20827837062 @default.
- W2082783706 hasOpenAccess W2082783706 @default.
- W2082783706 hasPrimaryLocation W20827837061 @default.
- W2082783706 hasRelatedWork W1897937419 @default.
- W2082783706 hasRelatedWork W1986534443 @default.
- W2082783706 hasRelatedWork W1996011126 @default.
- W2082783706 hasRelatedWork W1998478394 @default.
- W2082783706 hasRelatedWork W2012938620 @default.
- W2082783706 hasRelatedWork W2067568333 @default.
- W2082783706 hasRelatedWork W2095574104 @default.
- W2082783706 hasRelatedWork W2129917384 @default.
- W2082783706 hasRelatedWork W2514520035 @default.
- W2082783706 hasRelatedWork W4385256210 @default.
- W2082783706 hasVolume "9" @default.
- W2082783706 isParatext "false" @default.
- W2082783706 isRetracted "false" @default.
- W2082783706 magId "2082783706" @default.
- W2082783706 workType "article" @default.