Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082784126> ?p ?o ?g. }
- W2082784126 abstract "Abstract A simple method for the detection of small populations of Pseudomonas fluorescens P.B8-1, containing the nptII gene of Tn5 as a unique marker, was applied to a Nyuzen paddy soil using cell extraction (indirect DNA extraction) and a “nested” polymerase chain reaction (PCR). This involved processing samples through a combination of a sucrose gradient centrifugation procedure to isolate bacterial cells, followed by cell lysis with proteinase K and CTAB (hexadecyltrimethyl ammonium bromide)-NaCl. This method allowed the extraction of DNA within about 6 h followed by amplification of DNA. The optimized “nested” PCR comprised a “2-step” PCR (45 cycles) using two 20-mer primers, followed by a “3-step” PCR (30 cycles) using two 26-mer primers which were internal to the first set. After the first PCR step was performed, the amplified DNA was detectable from the inoculated soil containing a minimum of 105 cfu g−1. However, the “nested” PCR procedure permitted the detection of amplified DNA fragments from inoculated non-sterile soils containing 1.3 × 101 cfu g−1. The application of this detection strategy was tested by monitoring the survival of P. fluorescens P.B8-1 in a non-sterile paddy soil during a 53-day period. The P.B8-1 population decreased in soils maintained at either 25 or 10°C after inoculation. After 53 days, samples of soil maintained at 10°C contained 102 cfu g−1 of P.B8-1 (as determined by selective plate count) and permitted amplification of DNA by the “nested” PCR. At the same time, P.B8-1 was not detected in soil maintained at 25°C by either method. The results obtained using this detection strategy suggest that it is highly applicable to monitoring the fate of genetically engineered microorganisms in natural paddy soils." @default.
- W2082784126 created "2016-06-24" @default.
- W2082784126 creator A5004962655 @default.
- W2082784126 creator A5008031071 @default.
- W2082784126 creator A5008160990 @default.
- W2082784126 creator A5014611111 @default.
- W2082784126 creator A5045187129 @default.
- W2082784126 creator A5045866052 @default.
- W2082784126 creator A5054472689 @default.
- W2082784126 date "1995-02-01" @default.
- W2082784126 modified "2023-09-27" @default.
- W2082784126 title "Detection of genetically engineered microorganisms in paddy soil using a simple and rapid “nested” polymerase chain reaction method" @default.
- W2082784126 cites W1443667305 @default.
- W2082784126 cites W1494703094 @default.
- W2082784126 cites W1497810769 @default.
- W2082784126 cites W1498206429 @default.
- W2082784126 cites W1501954454 @default.
- W2082784126 cites W1511469905 @default.
- W2082784126 cites W1514657191 @default.
- W2082784126 cites W1548102296 @default.
- W2082784126 cites W1571452544 @default.
- W2082784126 cites W1583446458 @default.
- W2082784126 cites W1608514573 @default.
- W2082784126 cites W1873799311 @default.
- W2082784126 cites W1943150439 @default.
- W2082784126 cites W1968190169 @default.
- W2082784126 cites W2002587805 @default.
- W2082784126 cites W2060389943 @default.
- W2082784126 cites W2068809757 @default.
- W2082784126 cites W2079928468 @default.
- W2082784126 cites W2106955505 @default.
- W2082784126 cites W2111507183 @default.
- W2082784126 cites W2121731497 @default.
- W2082784126 cites W2137594675 @default.
- W2082784126 cites W2157783441 @default.
- W2082784126 cites W2160044108 @default.
- W2082784126 doi "https://doi.org/10.1016/0038-0717(94)00162-t" @default.
- W2082784126 hasPublicationYear "1995" @default.
- W2082784126 type Work @default.
- W2082784126 sameAs 2082784126 @default.
- W2082784126 citedByCount "21" @default.
- W2082784126 crossrefType "journal-article" @default.
- W2082784126 hasAuthorship W2082784126A5004962655 @default.
- W2082784126 hasAuthorship W2082784126A5008031071 @default.
- W2082784126 hasAuthorship W2082784126A5008160990 @default.
- W2082784126 hasAuthorship W2082784126A5014611111 @default.
- W2082784126 hasAuthorship W2082784126A5045187129 @default.
- W2082784126 hasAuthorship W2082784126A5045866052 @default.
- W2082784126 hasAuthorship W2082784126A5054472689 @default.
- W2082784126 hasConcept C104317684 @default.
- W2082784126 hasConcept C144024400 @default.
- W2082784126 hasConcept C149923435 @default.
- W2082784126 hasConcept C153911025 @default.
- W2082784126 hasConcept C157431215 @default.
- W2082784126 hasConcept C185592680 @default.
- W2082784126 hasConcept C190431759 @default.
- W2082784126 hasConcept C2776835041 @default.
- W2082784126 hasConcept C2908647359 @default.
- W2082784126 hasConcept C43617362 @default.
- W2082784126 hasConcept C4725764 @default.
- W2082784126 hasConcept C49105822 @default.
- W2082784126 hasConcept C523546767 @default.
- W2082784126 hasConcept C54355233 @default.
- W2082784126 hasConcept C552990157 @default.
- W2082784126 hasConcept C55493867 @default.
- W2082784126 hasConcept C57409179 @default.
- W2082784126 hasConcept C7623868 @default.
- W2082784126 hasConcept C78063203 @default.
- W2082784126 hasConcept C86803240 @default.
- W2082784126 hasConcept C89423630 @default.
- W2082784126 hasConceptScore W2082784126C104317684 @default.
- W2082784126 hasConceptScore W2082784126C144024400 @default.
- W2082784126 hasConceptScore W2082784126C149923435 @default.
- W2082784126 hasConceptScore W2082784126C153911025 @default.
- W2082784126 hasConceptScore W2082784126C157431215 @default.
- W2082784126 hasConceptScore W2082784126C185592680 @default.
- W2082784126 hasConceptScore W2082784126C190431759 @default.
- W2082784126 hasConceptScore W2082784126C2776835041 @default.
- W2082784126 hasConceptScore W2082784126C2908647359 @default.
- W2082784126 hasConceptScore W2082784126C43617362 @default.
- W2082784126 hasConceptScore W2082784126C4725764 @default.
- W2082784126 hasConceptScore W2082784126C49105822 @default.
- W2082784126 hasConceptScore W2082784126C523546767 @default.
- W2082784126 hasConceptScore W2082784126C54355233 @default.
- W2082784126 hasConceptScore W2082784126C552990157 @default.
- W2082784126 hasConceptScore W2082784126C55493867 @default.
- W2082784126 hasConceptScore W2082784126C57409179 @default.
- W2082784126 hasConceptScore W2082784126C7623868 @default.
- W2082784126 hasConceptScore W2082784126C78063203 @default.
- W2082784126 hasConceptScore W2082784126C86803240 @default.
- W2082784126 hasConceptScore W2082784126C89423630 @default.
- W2082784126 hasLocation W20827841261 @default.
- W2082784126 hasOpenAccess W2082784126 @default.
- W2082784126 hasPrimaryLocation W20827841261 @default.
- W2082784126 hasRelatedWork W1508277920 @default.
- W2082784126 hasRelatedWork W1528396837 @default.
- W2082784126 hasRelatedWork W1577668470 @default.
- W2082784126 hasRelatedWork W1577853014 @default.
- W2082784126 hasRelatedWork W1973069858 @default.
- W2082784126 hasRelatedWork W1975044179 @default.