Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082837141> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2082837141 endingPage "111" @default.
- W2082837141 startingPage "111" @default.
- W2082837141 abstract "The posttranslational processing of SI was investigated in organ culture of human intestinal biopsies labelled with 35S-methionine and immunoprecipitated with monoclonal antibodies. SI is synthesized as a high mannose precursor molecule of Mr 210,000 which is slowly converted in the Golgi to the complex glycosylated form of Mr 245,000. The half-time for the conversion of the Mr 210,000 to the Mr 245,000 is about 2 h. Owing to the absence of pancreatic secretions in the culture medium, only pro-SI and neither of the subunits S or I can be identified by SDS-PAGE. Trypsin, when added to homogenates from biosynthetically labelled biopsies, cleaved pro-SI to its subunits. Elastase and chymotrypsin were not effective. Based on this finding the transit time of pro-SI from the Golgi to the brush border membrane was estimated. In the presence of trypsin, the appearance of S and I and the decrease in the labelling intensity of pro-SI was observed after 2 h of chase. This is indicative for a membrane insertion of pro-SI and its exposure to trypsin in the culture medium. The half-time for the conversion of pro-SI by the aid of trypsin to S and I was between 30 min and 1 h which corresponds to the transit post Golgi-time." @default.
- W2082837141 created "2016-06-24" @default.
- W2082837141 creator A5001086890 @default.
- W2082837141 creator A5020009807 @default.
- W2082837141 creator A5054114735 @default.
- W2082837141 date "1987-07-01" @default.
- W2082837141 modified "2023-09-25" @default.
- W2082837141 title "94. KINETICS OF THE PROCESSING OF NEWLY SYNTHESIZED SUCRASE-ISOMALTASE (SI) IN ORGAN CULTURES OF HUMAN INTESTINAL BIOPSIES" @default.
- W2082837141 doi "https://doi.org/10.1203/00006450-198707000-00115" @default.
- W2082837141 hasPublicationYear "1987" @default.
- W2082837141 type Work @default.
- W2082837141 sameAs 2082837141 @default.
- W2082837141 citedByCount "2" @default.
- W2082837141 countsByYear W20828371412012 @default.
- W2082837141 crossrefType "journal-article" @default.
- W2082837141 hasAuthorship W2082837141A5001086890 @default.
- W2082837141 hasAuthorship W2082837141A5020009807 @default.
- W2082837141 hasAuthorship W2082837141A5054114735 @default.
- W2082837141 hasBestOaLocation W20828371411 @default.
- W2082837141 hasConcept C119062480 @default.
- W2082837141 hasConcept C130316041 @default.
- W2082837141 hasConcept C1491633281 @default.
- W2082837141 hasConcept C153911025 @default.
- W2082837141 hasConcept C159654299 @default.
- W2082837141 hasConcept C181199279 @default.
- W2082837141 hasConcept C185592680 @default.
- W2082837141 hasConcept C203014093 @default.
- W2082837141 hasConcept C2778401398 @default.
- W2082837141 hasConcept C2780340462 @default.
- W2082837141 hasConcept C2780912031 @default.
- W2082837141 hasConcept C41625074 @default.
- W2082837141 hasConcept C515207424 @default.
- W2082837141 hasConcept C5426402 @default.
- W2082837141 hasConcept C542903549 @default.
- W2082837141 hasConcept C55493867 @default.
- W2082837141 hasConcept C86803240 @default.
- W2082837141 hasConceptScore W2082837141C119062480 @default.
- W2082837141 hasConceptScore W2082837141C130316041 @default.
- W2082837141 hasConceptScore W2082837141C1491633281 @default.
- W2082837141 hasConceptScore W2082837141C153911025 @default.
- W2082837141 hasConceptScore W2082837141C159654299 @default.
- W2082837141 hasConceptScore W2082837141C181199279 @default.
- W2082837141 hasConceptScore W2082837141C185592680 @default.
- W2082837141 hasConceptScore W2082837141C203014093 @default.
- W2082837141 hasConceptScore W2082837141C2778401398 @default.
- W2082837141 hasConceptScore W2082837141C2780340462 @default.
- W2082837141 hasConceptScore W2082837141C2780912031 @default.
- W2082837141 hasConceptScore W2082837141C41625074 @default.
- W2082837141 hasConceptScore W2082837141C515207424 @default.
- W2082837141 hasConceptScore W2082837141C5426402 @default.
- W2082837141 hasConceptScore W2082837141C542903549 @default.
- W2082837141 hasConceptScore W2082837141C55493867 @default.
- W2082837141 hasConceptScore W2082837141C86803240 @default.
- W2082837141 hasIssue "1" @default.
- W2082837141 hasLocation W20828371411 @default.
- W2082837141 hasOpenAccess W2082837141 @default.
- W2082837141 hasPrimaryLocation W20828371411 @default.
- W2082837141 hasRelatedWork W1526478937 @default.
- W2082837141 hasRelatedWork W1993641094 @default.
- W2082837141 hasRelatedWork W1993929088 @default.
- W2082837141 hasRelatedWork W2007311491 @default.
- W2082837141 hasRelatedWork W2042857927 @default.
- W2082837141 hasRelatedWork W2053862149 @default.
- W2082837141 hasRelatedWork W2067981983 @default.
- W2082837141 hasRelatedWork W2069367789 @default.
- W2082837141 hasRelatedWork W2082837141 @default.
- W2082837141 hasRelatedWork W2118318665 @default.
- W2082837141 hasVolume "22" @default.
- W2082837141 isParatext "false" @default.
- W2082837141 isRetracted "false" @default.
- W2082837141 magId "2082837141" @default.
- W2082837141 workType "article" @default.