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- W2082901597 abstract "Mechanical strain has been shown to induce proliferation in neonatal vascular smooth muscle (VSM) cells. We compared the effects of strain on cultured VSM cells from epicardial and resistance coronary arteries from adult sheep hearts. Cells were grown on wells with silicone-elastomer bottoms, and subjected to strain (60 cycles/min) using a vacuum actuated strain device. 3H-thymidine incorporation was used as a measure of DNA synthesis. Strain induced increase in DNA synthesis was 1.9 fold higher in cells obtained from resistance arteries, compared to cells obtained from the left anterior descending (LAD) coronary artery. To gain further insights into the effects of strain on coronary artery VSM cells, we studied the time course of the strain-induced increase in DNA synthesis in cultured VSM cells from the LAD. We observed an increase in 3H-thymidine incorporation in response to strain with a peak at 18 hours, identical to the time to peak effect obtained in response to exogenous growth factors (PDGF and basic FGF), consistent with the continual rapid release of cellular growth factors from strained cells. Supernatant medium obtained from these cells induced a 2.01 fold increase in DNA synthesis in unstretched coronary VSM cells. Since TGF-βand basic FGF are both reported to be present in VSM cells, we explored the potential role of these factors in the strain induced response. Supernatant from LAD cells induced an inhibitory response in mink lung epithelial cells, suggesting the presence of TGF-βin the medium. In cultured LAD cells that had undergone multiple replications, the proliferative response was abolished by monoclonal antibodies to basic FGF. We conclude that mechanical strain is an important stimulus to proliferation in coronary VSM cells derived from both epicardial and resistance coronary arteries. In LAD cells, the increase in DNA synthesis may be mediated via release of autocrine growth factors, specifically TGF-βand/or basic FGF." @default.
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- W2082901597 date "1995-02-01" @default.
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- W2082901597 title "966-43 Mechanical Strain-induced Proliferation in Vascular Smooth Muscle Cells from Coronary Conductance and Resistance Arteries" @default.
- W2082901597 doi "https://doi.org/10.1016/0735-1097(95)92391-h" @default.
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