FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082911360> ?p ?o ?g. }

• W2082911360 endingPage "4877" @default.
• W2082911360 startingPage "4877" @default.
• W2082911360 abstract "We have developed a method for the detection of genetic markers associated with high pathogenicity in influenza. The assay consists of an array of 5′-thiolated ssDNA oligonucleotides immobilized on the surface of a Ag nanorod substrate that serve as capture probes for the detection of synthetic RNA sequences coding for a genetic mutation in the influenza PB1-F2 protein. Hybridization of the DNA probes to their complementary RNA sequences was detected using surface-enhanced Raman spectroscopy (SERS). Multivariate statistical analysis was used to differentiate the spectra of the complementary DNA probe-RNA target hybrids from those of the non-complementary DNA probes containing a single base pair polymorphism. Hierarchical cluster analysis (HCA) was able to distinguish with 100% accuracy the spectra of the complementary DNA probe–RNA target from the spectra of the immobilized DNA probes alone, or the DNA probes incubated with non-complementary RNA sequences. Linearity of response and limits of sensitivity of the SERS-based assays were determined using a partial least squares (PLS) regression model; detection limits computed by PLS was determined to be ∼10 nM. The binding affinity of the DNA probes to their complementary RNA sequences was confirmed using enzyme-linked immunosorbent assay (ELISA); however, the detection limits observed using ELISA were approximately 10× higher (∼100 nM) than those determined by PLS analysis of the SERS spectra." @default.
• W2082911360 created "2016-06-24" @default.
• W2082911360 creator A5004669309 @default.
• W2082911360 creator A5066152908 @default.
• W2082911360 date "2013-01-01" @default.
• W2082911360 modified "2023-09-26" @default.
• W2082911360 title "Detection of genetic markers related to high pathogenicity in influenza by SERS" @default.
• W2082911360 cites W1483301458 @default.
• W2082911360 cites W1969670668 @default.
• W2082911360 cites W1976223177 @default.
• W2082911360 cites W1985043444 @default.
• W2082911360 cites W1985223811 @default.
• W2082911360 cites W1991617986 @default.
• W2082911360 cites W1992521903 @default.
• W2082911360 cites W1996311210 @default.
• W2082911360 cites W1997644350 @default.
• W2082911360 cites W2000579609 @default.
• W2082911360 cites W2001893591 @default.
• W2082911360 cites W2005425942 @default.
• W2082911360 cites W2006766146 @default.
• W2082911360 cites W2013837407 @default.
• W2082911360 cites W2017333937 @default.
• W2082911360 cites W2020718365 @default.
• W2082911360 cites W2026391844 @default.
• W2082911360 cites W2031336395 @default.
• W2082911360 cites W2036108754 @default.
• W2082911360 cites W2040533212 @default.
• W2082911360 cites W2042480859 @default.
• W2082911360 cites W2043312520 @default.
• W2082911360 cites W2043468628 @default.
• W2082911360 cites W2043697173 @default.
• W2082911360 cites W2045083682 @default.
• W2082911360 cites W2047976942 @default.
• W2082911360 cites W2067567649 @default.
• W2082911360 cites W2077190295 @default.
• W2082911360 cites W2077448704 @default.
• W2082911360 cites W2086727917 @default.
• W2082911360 cites W2090043560 @default.
• W2082911360 cites W2092380725 @default.
• W2082911360 cites W2095086942 @default.
• W2082911360 cites W2098782379 @default.
• W2082911360 cites W2104024911 @default.
• W2082911360 cites W2111785766 @default.
• W2082911360 cites W2116682907 @default.
• W2082911360 cites W2123075456 @default.
• W2082911360 cites W2130251737 @default.
• W2082911360 cites W2133318493 @default.
• W2082911360 cites W2137146240 @default.
• W2082911360 cites W2139841187 @default.
• W2082911360 cites W2142480654 @default.
• W2082911360 cites W2149578519 @default.
• W2082911360 cites W2150193552 @default.
• W2082911360 cites W2152170523 @default.
• W2082911360 cites W2153692349 @default.
• W2082911360 cites W2154372877 @default.
• W2082911360 cites W2154487982 @default.
• W2082911360 cites W2164583936 @default.
• W2082911360 cites W2323928941 @default.
• W2082911360 cites W3131735448 @default.
• W2082911360 doi "https://doi.org/10.1039/c3an00774j" @default.
• W2082911360 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3767290" @default.
• W2082911360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23833767" @default.
• W2082911360 hasPublicationYear "2013" @default.
• W2082911360 type Work @default.
• W2082911360 sameAs 2082911360 @default.
• W2082911360 citedByCount "20" @default.
• W2082911360 countsByYear W20829113602014 @default.
• W2082911360 countsByYear W20829113602015 @default.
• W2082911360 countsByYear W20829113602017 @default.
• W2082911360 countsByYear W20829113602018 @default.
• W2082911360 countsByYear W20829113602019 @default.
• W2082911360 countsByYear W20829113602020 @default.
• W2082911360 countsByYear W20829113602021 @default.
• W2082911360 countsByYear W20829113602022 @default.
• W2082911360 countsByYear W20829113602023 @default.
• W2082911360 crossrefType "journal-article" @default.
• W2082911360 hasAuthorship W2082911360A5004669309 @default.
• W2082911360 hasAuthorship W2082911360A5066152908 @default.
• W2082911360 hasBestOaLocation W20829113602 @default.
• W2082911360 hasConcept C104317684 @default.
• W2082911360 hasConcept C119128265 @default.
• W2082911360 hasConcept C120665830 @default.
• W2082911360 hasConcept C121332964 @default.
• W2082911360 hasConcept C129312508 @default.
• W2082911360 hasConcept C130197536 @default.
• W2082911360 hasConcept C153911025 @default.
• W2082911360 hasConcept C169573571 @default.
• W2082911360 hasConcept C185592680 @default.
• W2082911360 hasConcept C2777790068 @default.
• W2082911360 hasConcept C40003534 @default.
• W2082911360 hasConcept C43617362 @default.
• W2082911360 hasConcept C552990157 @default.
• W2082911360 hasConcept C55493867 @default.
• W2082911360 hasConcept C67705224 @default.
• W2082911360 hasConcept C86803240 @default.
• W2082911360 hasConceptScore W2082911360C104317684 @default.
• W2082911360 hasConceptScore W2082911360C119128265 @default.
• W2082911360 hasConceptScore W2082911360C120665830 @default.

• next