Matches in SemOpenAlex for { <https://semopenalex.org/work/W2082912665> ?p ?o ?g. }
- W2082912665 endingPage "3395" @default.
- W2082912665 startingPage "3386" @default.
- W2082912665 abstract "The effects of low-pH treatments on DNA structure in polytene chromosomes of Chironomus thummi thummi have been studied by Raman microspectroscopy. Measurements were carried out on chromosomes at low pH and on chromosomes reneutralized after a short exposure to low pH. Protonation of adenine residues and subsequent unpairing of adenine (A) and thymine (T) were found to commence already above pH 3.6 and are completed at pH 2.2. Protonation of the cytosine-guanine base pair also starts above pH 3.6. It leads to an intermediate protonated, most likely Hoogsteen-type, guanine-cytosine base pair. Unpairing of G and C residues starts between pH 2.6 and 2.2 and continues below pH 2.2. Brief treatment of chromosomes at a pH < or = 2.2, i.e., at a pH where both AT and GC base pairs are disrupted, was found to lead to irreversible changes in DNA conformation upon return to neutral pH. These were most pronounced after treatment with 45% acetic acid. More than 10% of the A and T residues was found to have adopted a non-B-DNA conformation. Evidence was found for denaturation of the B-form backbone. The amount of protein extracted from the chromosomes was strongly pH-dependent. Treatment at pH 3.6 did not cause noticeable protein extraction, while treatment with 45% acetic acid extracted more than 50% (by weight) of the chromosomal proteins." @default.
- W2082912665 created "2016-06-24" @default.
- W2082912665 creator A5003623266 @default.
- W2082912665 creator A5005496508 @default.
- W2082912665 creator A5009958441 @default.
- W2082912665 creator A5010280330 @default.
- W2082912665 creator A5020057579 @default.
- W2082912665 creator A5038585473 @default.
- W2082912665 date "1994-03-22" @default.
- W2082912665 modified "2023-10-18" @default.
- W2082912665 title "Raman Microspectroscopic Study of Low-pH-Induced Changes in DNA Structure of Polytene Chromosomes" @default.
- W2082912665 cites W104862819 @default.
- W2082912665 cites W1471571550 @default.
- W2082912665 cites W1510061653 @default.
- W2082912665 cites W1570668711 @default.
- W2082912665 cites W1964606787 @default.
- W2082912665 cites W1968286102 @default.
- W2082912665 cites W1970585122 @default.
- W2082912665 cites W1971675894 @default.
- W2082912665 cites W1972838830 @default.
- W2082912665 cites W1973503110 @default.
- W2082912665 cites W1973775164 @default.
- W2082912665 cites W1978888013 @default.
- W2082912665 cites W1979412187 @default.
- W2082912665 cites W1986614682 @default.
- W2082912665 cites W1990154423 @default.
- W2082912665 cites W1990471234 @default.
- W2082912665 cites W1994716553 @default.
- W2082912665 cites W1996254913 @default.
- W2082912665 cites W1999217903 @default.
- W2082912665 cites W2002149186 @default.
- W2082912665 cites W2002449418 @default.
- W2082912665 cites W2005282401 @default.
- W2082912665 cites W2013202743 @default.
- W2082912665 cites W2015787992 @default.
- W2082912665 cites W2020378172 @default.
- W2082912665 cites W2023561500 @default.
- W2082912665 cites W2024236380 @default.
- W2082912665 cites W2030901249 @default.
- W2082912665 cites W2031170923 @default.
- W2082912665 cites W2037478998 @default.
- W2082912665 cites W2038669421 @default.
- W2082912665 cites W2039042911 @default.
- W2082912665 cites W2043246234 @default.
- W2082912665 cites W2050822975 @default.
- W2082912665 cites W2058673131 @default.
- W2082912665 cites W2060807003 @default.
- W2082912665 cites W2063102503 @default.
- W2082912665 cites W2063311279 @default.
- W2082912665 cites W2063496615 @default.
- W2082912665 cites W2064076654 @default.
- W2082912665 cites W2064393761 @default.
- W2082912665 cites W2067064915 @default.
- W2082912665 cites W2068803499 @default.
- W2082912665 cites W2074181493 @default.
- W2082912665 cites W2075700539 @default.
- W2082912665 cites W2075719295 @default.
- W2082912665 cites W2076460812 @default.
- W2082912665 cites W2078803436 @default.
- W2082912665 cites W2078986175 @default.
- W2082912665 cites W2080910018 @default.
- W2082912665 cites W2081446791 @default.
- W2082912665 cites W2082161139 @default.
- W2082912665 cites W2086015167 @default.
- W2082912665 cites W2089981004 @default.
- W2082912665 cites W2090930027 @default.
- W2082912665 cites W2091677739 @default.
- W2082912665 cites W2094137173 @default.
- W2082912665 cites W2097190875 @default.
- W2082912665 cites W2104711332 @default.
- W2082912665 cites W2107336540 @default.
- W2082912665 cites W2117128967 @default.
- W2082912665 cites W2123428556 @default.
- W2082912665 cites W2123786201 @default.
- W2082912665 cites W2130613535 @default.
- W2082912665 cites W2144091365 @default.
- W2082912665 cites W2148308569 @default.
- W2082912665 cites W2149329327 @default.
- W2082912665 cites W2156163511 @default.
- W2082912665 cites W2169200668 @default.
- W2082912665 cites W2170545389 @default.
- W2082912665 cites W2175249821 @default.
- W2082912665 cites W2430370895 @default.
- W2082912665 cites W655500139 @default.
- W2082912665 doi "https://doi.org/10.1021/bi00177a032" @default.
- W2082912665 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8136376" @default.
- W2082912665 hasPublicationYear "1994" @default.
- W2082912665 type Work @default.
- W2082912665 sameAs 2082912665 @default.
- W2082912665 citedByCount "83" @default.
- W2082912665 countsByYear W20829126652012 @default.
- W2082912665 countsByYear W20829126652013 @default.
- W2082912665 countsByYear W20829126652014 @default.
- W2082912665 countsByYear W20829126652015 @default.
- W2082912665 countsByYear W20829126652016 @default.
- W2082912665 countsByYear W20829126652017 @default.
- W2082912665 countsByYear W20829126652018 @default.
- W2082912665 countsByYear W20829126652019 @default.