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- W2082984865 abstract "ABSTRACT Recently, artificial gene networks have been developed in synthetic biology to control gene expression and make organisms as controllable as robots. Here, I present an artificial posttranslational gene-silencing system based on the codon usage bias and low tRNA content corresponding to minor codons. I engineered the green fluorescent protein (GFP) gene to inhibit translation indirectly with the lowest-usage codons to monopolize various minor tRNAs ( lgfp ). The expression of lgfp interfered nonspecifically with the growth of Escherichia coli , Saccharomyces cerevisiae , human HeLa cervical cancer cells, MCF7 breast cancer cells, and HEK293 kidney cells, as well as phage and adenovirus expansion. Furthermore, insertion of lgfp downstream of a phage response promoter conferred phage resistance on E. coli . Such engineered gene silencers could act as components of biological networks capable of functioning with suitable promoters in E. coli , S. cerevisiae , and human cells to control gene expression. The results presented here show general suppressor artificial genes for live cells and viruses. This robust system provides a gene expression or cell growth control device for artificially synthesized gene networks." @default.
- W2082984865 created "2016-06-24" @default.
- W2082984865 creator A5080449029 @default.
- W2082984865 date "2015-04-01" @default.
- W2082984865 modified "2023-09-26" @default.
- W2082984865 title "Inducible Suppression of Global Translation by Overuse of Rare Codons" @default.
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- W2082984865 doi "https://doi.org/10.1128/aem.03708-14" @default.
- W2082984865 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4357939" @default.
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