Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083021997> ?p ?o ?g. }
- W2083021997 endingPage "1298" @default.
- W2083021997 startingPage "1289" @default.
- W2083021997 abstract "beta-lactam antibiotic therapies are commonly challenged by the hydrolytic activities of beta-lactamases in bacteria. These enzymes have been grouped into four classes: A, B, C, and D. Class B beta-lactamases are zinc dependent, and enzymes of classes A, C, and D are transiently acylated on a serine residue in the course of the turnover chemistry. While class A and C beta-lactamases have been extensively characterized by biochemical and structural methods, class D enzymes remain the least studied despite their increasing importance in the clinic.The crystal structure of the OXA10 class D beta-lactamase has been solved to 1.66 A resolution from a gold derivative and MAD phasing. This structure reveals that beta-lactamases from classes D and A, despite very poor sequence similarity, share a similar overall fold. An additional beta strand in OXA10 mediates the association into dimers characterized by analytical ultracentrifugation. Major differences are found when comparing the molecular details of the active site of this class D enzyme to the corresponding regions in class A and C beta-lactamases. In the native structure of the OXA10 enzyme solved to 1.8 A, Lys-70 is carbamylated.Several features were revealed by this study: the dimeric structure of the OXA10 beta-lactamase, an extension of the substrate binding site which suggests that class D enzymes may bind other substrates beside beta-lactams, and carbamylation of the active site Lys-70 residue. The CO2-dependent activity of the OXA10 enzyme and the kinetic properties of the natural OXA17 mutant protein suggest possible relationships between carbamylation, inhibition of the enzyme by anions, and biphasic behavior of the enzyme." @default.
- W2083021997 created "2016-06-24" @default.
- W2083021997 creator A5013812375 @default.
- W2083021997 creator A5033881268 @default.
- W2083021997 creator A5043351423 @default.
- W2083021997 creator A5059252438 @default.
- W2083021997 creator A5066210282 @default.
- W2083021997 creator A5067999938 @default.
- W2083021997 creator A5086433612 @default.
- W2083021997 date "2000-12-01" @default.
- W2083021997 modified "2023-09-25" @default.
- W2083021997 title "Insights into Class D β-Lactamases Are Revealed by the Crystal Structure of the OXA10 Enzyme from Pseudomonas aeruginosa" @default.
- W2083021997 cites W1581506296 @default.
- W2083021997 cites W1591680814 @default.
- W2083021997 cites W1820365410 @default.
- W2083021997 cites W1870016320 @default.
- W2083021997 cites W1908427699 @default.
- W2083021997 cites W1965277349 @default.
- W2083021997 cites W1969181216 @default.
- W2083021997 cites W1980595924 @default.
- W2083021997 cites W1985629904 @default.
- W2083021997 cites W198863919 @default.
- W2083021997 cites W1993404146 @default.
- W2083021997 cites W1996960109 @default.
- W2083021997 cites W2001641653 @default.
- W2083021997 cites W2002664017 @default.
- W2083021997 cites W2014391628 @default.
- W2083021997 cites W2015304573 @default.
- W2083021997 cites W2026219411 @default.
- W2083021997 cites W2031232438 @default.
- W2083021997 cites W2031772330 @default.
- W2083021997 cites W2036380814 @default.
- W2083021997 cites W2038840577 @default.
- W2083021997 cites W2039388361 @default.
- W2083021997 cites W2047709322 @default.
- W2083021997 cites W2057958258 @default.
- W2083021997 cites W2074112877 @default.
- W2083021997 cites W2081962393 @default.
- W2083021997 cites W2089658775 @default.
- W2083021997 cites W2091679105 @default.
- W2083021997 cites W2097382368 @default.
- W2083021997 cites W2101334024 @default.
- W2083021997 cites W2111516721 @default.
- W2083021997 cites W2113030266 @default.
- W2083021997 cites W2122102756 @default.
- W2083021997 cites W2128577323 @default.
- W2083021997 cites W2131950041 @default.
- W2083021997 cites W2133829625 @default.
- W2083021997 cites W2134253205 @default.
- W2083021997 cites W2134352676 @default.
- W2083021997 cites W2139658982 @default.
- W2083021997 cites W2141254180 @default.
- W2083021997 cites W2143874510 @default.
- W2083021997 cites W2147872249 @default.
- W2083021997 cites W2154771379 @default.
- W2083021997 cites W2158919479 @default.
- W2083021997 cites W2163623511 @default.
- W2083021997 cites W2165414378 @default.
- W2083021997 cites W2280344573 @default.
- W2083021997 cites W3023926866 @default.
- W2083021997 cites W4232928039 @default.
- W2083021997 cites W4242135481 @default.
- W2083021997 doi "https://doi.org/10.1016/s0969-2126(00)00534-7" @default.
- W2083021997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11188693" @default.
- W2083021997 hasPublicationYear "2000" @default.
- W2083021997 type Work @default.
- W2083021997 sameAs 2083021997 @default.
- W2083021997 citedByCount "112" @default.
- W2083021997 countsByYear W20830219972012 @default.
- W2083021997 countsByYear W20830219972013 @default.
- W2083021997 countsByYear W20830219972014 @default.
- W2083021997 countsByYear W20830219972015 @default.
- W2083021997 countsByYear W20830219972016 @default.
- W2083021997 countsByYear W20830219972017 @default.
- W2083021997 countsByYear W20830219972018 @default.
- W2083021997 countsByYear W20830219972019 @default.
- W2083021997 countsByYear W20830219972020 @default.
- W2083021997 countsByYear W20830219972021 @default.
- W2083021997 countsByYear W20830219972022 @default.
- W2083021997 countsByYear W20830219972023 @default.
- W2083021997 crossrefType "journal-article" @default.
- W2083021997 hasAuthorship W2083021997A5013812375 @default.
- W2083021997 hasAuthorship W2083021997A5033881268 @default.
- W2083021997 hasAuthorship W2083021997A5043351423 @default.
- W2083021997 hasAuthorship W2083021997A5059252438 @default.
- W2083021997 hasAuthorship W2083021997A5066210282 @default.
- W2083021997 hasAuthorship W2083021997A5067999938 @default.
- W2083021997 hasAuthorship W2083021997A5086433612 @default.
- W2083021997 hasBestOaLocation W20830219971 @default.
- W2083021997 hasConcept C181199279 @default.
- W2083021997 hasConcept C185592680 @default.
- W2083021997 hasConcept C2776414213 @default.
- W2083021997 hasConcept C2780120296 @default.
- W2083021997 hasConcept C2781338088 @default.
- W2083021997 hasConcept C41183919 @default.
- W2083021997 hasConcept C55493867 @default.
- W2083021997 hasConcept C71240020 @default.
- W2083021997 hasConceptScore W2083021997C181199279 @default.