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- W2083048982 abstract "Understanding the structure-function relationships at cellular, circuit, and organ-wide scale requires 3D anatomical and phenotypical maps, currently unavailable for many organs across species. At the root of this knowledge gap is the absence of a method that enables whole-organ imaging. Herein, we present techniques for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically transparent, thereby exposing their cellular structure with intact connectivity. We describe PACT (passive clarity technique), a protocol for passive tissue clearing and immunostaining of intact organs; RIMS (refractive index matching solution), a mounting media for imaging thick tissue; and PARS (perfusion-assisted agent release in situ), a method for whole-body clearing and immunolabeling. We show that in rodents PACT, RIMS, and PARS are compatible with endogenous-fluorescence, immunohistochemistry, RNA single-molecule FISH, long-term storage, and microscopy with cellular and subcellular resolution. These methods are applicable for high-resolution, high-content mapping and phenotyping of normal and pathological elements within intact organs and bodies." @default.
- W2083048982 created "2016-06-24" @default.
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- W2083048982 date "2014-08-01" @default.
- W2083048982 modified "2023-10-18" @default.
- W2083048982 title "Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing" @default.
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- W2083048982 doi "https://doi.org/10.1016/j.cell.2014.07.017" @default.
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