Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083100997> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2083100997 endingPage "285a" @default.
- W2083100997 startingPage "285a" @default.
- W2083100997 abstract "Bacterial membranes present solid-ordered/liquid-disordered (so/ld) cooperative melting event close to physiological temperature. The cellular advantage of this thermotropic melting event is yet to be determined. We show that this thermal behavior provides resistance towards a membrane active antibacterial agent. Phospholipase A2 type IIA (sPLA2-IIA) is a hydrolytic enzyme which presents antibacterial properties towards Gram positive bacteria. The enzyme has higher activity in ld phase compared to so phase in anionic membranes. We show that the lipid phase behavior of Staphylococcus aureus (S.aureus) membranes as measured by FTIR modulates sPLA2-IIA by inducing a sharp drop in activity below the melting temperature of the membrane (centered at 15.3°C). The effects of sPLA2-IIA treatment on cell viability are also investigated. While above the main melting event viability drops to 20% of the initial CFU after treatment, below the main melting event cell viability only drops to 60% under the same treatment. This strongly suggests that cells in the solid-ordered phase are better adapted to survive the enzymatic insult. These results led us to explore if a subpopulation of S.aureus enriched in ordered lipids can be selected after repeated treatment with sPLA2-IIA at 37°C. After selecting for resistance at 37°C we measured growth curves, membrane order, and cell viability as a function of treatment temperature. The results suggest that at 37°C there is a bacterial subpopulation with increased membrane rigidity that insures survival of the colony to an insult by sPLA2-IIA. This subpopulation also presents a longer latency period which can be explained by the increased presence in ordered lipids, which are known to inhibit cell division. Even if the growth conditions are not optimal, the presence of this subpopulation ensures survival from the antibacterial insult." @default.
- W2083100997 created "2016-06-24" @default.
- W2083100997 creator A5003741342 @default.
- W2083100997 creator A5025064657 @default.
- W2083100997 creator A5049468019 @default.
- W2083100997 creator A5053630014 @default.
- W2083100997 creator A5056844577 @default.
- W2083100997 creator A5058506964 @default.
- W2083100997 creator A5060971568 @default.
- W2083100997 date "2010-01-01" @default.
- W2083100997 modified "2023-10-16" @default.
- W2083100997 title "Staphylococcus aureus Enriched in Ordered Lipids Present Resistance Towards the Antibacterial Agent sPLA2-IIA: An Unusual Mechanism to Survive" @default.
- W2083100997 doi "https://doi.org/10.1016/j.bpj.2009.12.1555" @default.
- W2083100997 hasPublicationYear "2010" @default.
- W2083100997 type Work @default.
- W2083100997 sameAs 2083100997 @default.
- W2083100997 citedByCount "1" @default.
- W2083100997 countsByYear W20831009972023 @default.
- W2083100997 crossrefType "journal-article" @default.
- W2083100997 hasAuthorship W2083100997A5003741342 @default.
- W2083100997 hasAuthorship W2083100997A5025064657 @default.
- W2083100997 hasAuthorship W2083100997A5049468019 @default.
- W2083100997 hasAuthorship W2083100997A5053630014 @default.
- W2083100997 hasAuthorship W2083100997A5056844577 @default.
- W2083100997 hasAuthorship W2083100997A5058506964 @default.
- W2083100997 hasAuthorship W2083100997A5060971568 @default.
- W2083100997 hasBestOaLocation W20831009971 @default.
- W2083100997 hasConcept C12554922 @default.
- W2083100997 hasConcept C13530604 @default.
- W2083100997 hasConcept C1491633281 @default.
- W2083100997 hasConcept C17741926 @default.
- W2083100997 hasConcept C178790620 @default.
- W2083100997 hasConcept C181199279 @default.
- W2083100997 hasConcept C185592680 @default.
- W2083100997 hasConcept C19655278 @default.
- W2083100997 hasConcept C2778106830 @default.
- W2083100997 hasConcept C2779489039 @default.
- W2083100997 hasConcept C41625074 @default.
- W2083100997 hasConcept C523546767 @default.
- W2083100997 hasConcept C53227056 @default.
- W2083100997 hasConcept C54355233 @default.
- W2083100997 hasConcept C55493867 @default.
- W2083100997 hasConcept C86803240 @default.
- W2083100997 hasConcept C89423630 @default.
- W2083100997 hasConceptScore W2083100997C12554922 @default.
- W2083100997 hasConceptScore W2083100997C13530604 @default.
- W2083100997 hasConceptScore W2083100997C1491633281 @default.
- W2083100997 hasConceptScore W2083100997C17741926 @default.
- W2083100997 hasConceptScore W2083100997C178790620 @default.
- W2083100997 hasConceptScore W2083100997C181199279 @default.
- W2083100997 hasConceptScore W2083100997C185592680 @default.
- W2083100997 hasConceptScore W2083100997C19655278 @default.
- W2083100997 hasConceptScore W2083100997C2778106830 @default.
- W2083100997 hasConceptScore W2083100997C2779489039 @default.
- W2083100997 hasConceptScore W2083100997C41625074 @default.
- W2083100997 hasConceptScore W2083100997C523546767 @default.
- W2083100997 hasConceptScore W2083100997C53227056 @default.
- W2083100997 hasConceptScore W2083100997C54355233 @default.
- W2083100997 hasConceptScore W2083100997C55493867 @default.
- W2083100997 hasConceptScore W2083100997C86803240 @default.
- W2083100997 hasConceptScore W2083100997C89423630 @default.
- W2083100997 hasIssue "3" @default.
- W2083100997 hasLocation W20831009971 @default.
- W2083100997 hasOpenAccess W2083100997 @default.
- W2083100997 hasPrimaryLocation W20831009971 @default.
- W2083100997 hasRelatedWork W1018601499 @default.
- W2083100997 hasRelatedWork W2069222261 @default.
- W2083100997 hasRelatedWork W2077379206 @default.
- W2083100997 hasRelatedWork W2094643200 @default.
- W2083100997 hasRelatedWork W2381880186 @default.
- W2083100997 hasRelatedWork W2416702355 @default.
- W2083100997 hasRelatedWork W2553424334 @default.
- W2083100997 hasRelatedWork W2593466179 @default.
- W2083100997 hasRelatedWork W3146299994 @default.
- W2083100997 hasRelatedWork W4323982057 @default.
- W2083100997 hasVolume "98" @default.
- W2083100997 isParatext "false" @default.
- W2083100997 isRetracted "false" @default.
- W2083100997 magId "2083100997" @default.
- W2083100997 workType "article" @default.