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- W2083133003 abstract "A method for fractionation of barley chromatin was developed. A soluble fraction, eluted from micrococcal nuclease-digested nuclei, was recovered and studied in comparison with the low ionic strength soluble bulk chromatin obtained after lysis of nuclei. The eluted fraction contained mainly mononucleosomes with less than 10% dinucleosomes. It was highly micrococcal nuclease-sensitive, totally depleted in the highest molecular mass H1 subspecies, and enriched both in the lowest Mr proteins and in other acid-soluble proteins. The soluble bulk chromatin exhibited protein and DNA contents analogous to those of the low ionic strength insoluble fraction. In addition, high-mobility-group proteins purification was carried out. Four species were identified. They were almost exclusively localized in the highly micrococcal nuclease-sensitive fraction." @default.
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- W2083133003 date "1984-04-01" @default.
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- W2083133003 title "Study of a chromatin domain different from bulk chromatin in barley nuclei" @default.
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- W2083133003 doi "https://doi.org/10.1016/0167-4781(84)90095-2" @default.
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