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• W2083157319 abstract "The synthesis of the novel, fluorescent 3-aminobiphenyl-C-nucleoside M as well as the corresponding building block for oligodeoxynucleotide synthesis is described. M was incorporated into oligodeoxynucleotides via standard phosphoramidite chemistry and the thermal stabilities of duplexes with one and three consecutive M–M, M–P, and M–O pairs, where P denotes an unmodified biphenyl C-nucleoside and O a 3,5-dinitrobiphenyl-C-nucleoside, were determined. It was found that duplexes containing three consecutive M–O pairs were the most stable in the series, notably more stable than a duplex with one additional natural G–C pair instead of the modified residues. Furthermore it was found that the fluorescence of M is efficiently quenched in a duplex when placed opposite to the dinitrophenyl unit O. Thus M and O constitute a novel fluorophore/quencher pair that is orthogonal to natural base pairs in its recognition properties enabling its use as highly specific tags with reporting properties." @default.
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• W2083157319 date "2007-04-01" @default.
• W2083157319 modified "2023-10-10" @default.
• W2083157319 title "A fluorescence-quencher pair for DNA hybridization studies based on hydrophobic base surrogates" @default.
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• W2083157319 doi "https://doi.org/10.1016/j.tet.2006.12.095" @default.
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