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- W2083233570 abstract "Abstract A nucleotide stretch located upstream of the MRAS3 gene of the zygomycete Mucor racemosus was found to hybridize to multiple restriction fragments of genomic DNA of the ascomycete Podospora anserina . DNA of strains derived from different geographical locations revealed no major restriction length fragment polymorphisms. Also, no age-related polymorphisms were observed. The repetitive sequence is located on all of the physically fractionated Podospora chromosomes. Sequence analysis of the upstream sequence of MRAS3 and of a DNA-stretch from a genomic clone of P. anserina which hybridized to the Mucor probe revealed a region of sequence homology. This sequence is composed of alternating G and T residues [poly(dG-dT) or poly(GT)] of 76 bp in length and thus represents a simple repetitive sequence, or microsatellite. A synthetic oligonucleotide, (GT) 8 , corresponding to this sequence gave also rise to a complex hybridization pattern. Screening of genomic libraries of P. anserina revealed poly(GT)-stretches on about 5–7% of all clones. Finally, this repetitive sequence was identified in the genome of the filamentous fungi Sordaria macrospora and Curvularia lunata ." @default.
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- W2083233570 date "1996-03-01" @default.
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- W2083233570 title "Genome analysis of filamentous fungi: identification of a highly conserved simple repetitive sequence in different strains of Podospora anserina" @default.
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- W2083233570 doi "https://doi.org/10.1016/s0944-5013(96)80049-8" @default.
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