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- W2083258595 abstract "The design and performance of the filter holder which enables convenient preparations of volumes of up to a milliliter of large, unilamellar vesicles by extrusion (LUVETs) from multilamellar vesicles (MLVs) are described. The filter holder provides for back-and-forth passage of the sample between two syringes, a design that minimizes filter blockage, eliminates the need to change filters during LUVET preparation and reduces preparation time to a few minutes. Replicas of slam-frozen LUVETs in the electron microscope are unilamellar and reasonably homogeneous with an average diameter close to the pore size of the filters used to extrude them. Extrusion per se does not destabilize the vesicles, which trapped a fluorescent dye only when they were disrupted on freeze-thawing and during the first extrusion when most of the MLVs were apparently converted to LUVETS." @default.
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- W2083258595 date "1991-01-01" @default.
- W2083258595 modified "2023-10-16" @default.
- W2083258595 title "Small-volume extrusion apparatus for preparation of large, unilamellar vesicles" @default.
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- W2083258595 doi "https://doi.org/10.1016/0005-2736(91)90295-j" @default.
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