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- W2083302057 abstract "Currently used methods for the culture of mycobacteria from contaminated material were found to be unsatisfactory in an investigation of a possible environmental source of Mycobacterium avium-intracellulare-scrofulaceum (MAIS) infection in a New Zealand deer farm. Different combinations of established procedures were investigated using soil spiked with a laboratory strain of M. avium. The most successful combination involved mixing the soil in nutrient broth (pH 8mD0) containing Tween 80, incubating at 37°C for 1 h to germinate sporing contaminants, treatment for 24 h with 1% cetylpyridinium chloride, followed by washing and culture on Lowenstein-Jensen slopes and incubation at 37°C and 42°C in approximately 5% CO2 atmosphere. This procedure allowed good recovery of M. avium while successfully inhibiting saprophytic mycobacteria and other soil organisms, and was chosen to process the deer farm samples. No mycobacteria resembling the deer strains were found in these samples." @default.
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- W2083302057 date "1989-03-01" @default.
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- W2083302057 title "Methods used to investigate a possible environmental source ofMycobacterium avium-intracellulare-scrofulaceum(MAIS) infection in farmed deer" @default.
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- W2083302057 doi "https://doi.org/10.1111/j.1365-2672.1989.tb02472.x" @default.
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