Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083311205> ?p ?o ?g. }
- W2083311205 endingPage "61" @default.
- W2083311205 startingPage "55" @default.
- W2083311205 abstract "Vascular smooth muscle cell (VSMC) migration plays a pivotal role in the pathogenesis of arteriosclerosis, under influences of various mechanical factors. Thus, we examined whether static pressure promotes VSMC migration and if so, whether Rho-kinase is involved. Rat VSMCs were cultured on chambers coated on fibronectin, vitronectin, laminin, or type IV collagen, under pressure-free conditions and at 90 and 180 mm Hg. In monolayer-wounding assay, VSMC migration was significantly increased after 72 hours at 180 mm Hg on both fibronectin (11.3 ± 3.4-fold vs. pressure-free conditions) and vitronectin (10.6 ± 0.7-fold; both P < 0.05). In Boyden chamber assay, the VSMC migration was again significantly increased at 180 mm Hg on both fibronectin (4.0 ± 0.5-fold) and vitronectin (5.0 ± 0.8-fold; both P < 0.05). Neutralizing antibodies against β1-, β3- and β5-integrins, all of which play an important role in cell migration, significantly inhibited the pressure-promoted VSMC migration. Static pressure also significantly increased Rho-kinase activity in VSMC, as evaluated by the extent of phosphorylation of its downstream substrate, ezrin-radixin-moesin. Fasudil, a selective Rho-kinase inhibitor, significantly suppressed the pressure-promoted VSMC migration with reduced Rho-kinase activity. These results indicate that increased static pressure promotes VSMC migration through the integrin/Rho-kinase signaling, suggesting the therapeutic importance of this mechanism for the treatment of hypertensive vascular diseases." @default.
- W2083311205 created "2016-06-24" @default.
- W2083311205 creator A5009988205 @default.
- W2083311205 creator A5012622166 @default.
- W2083311205 creator A5012657143 @default.
- W2083311205 creator A5021433405 @default.
- W2083311205 creator A5037302816 @default.
- W2083311205 creator A5058362593 @default.
- W2083311205 creator A5062835440 @default.
- W2083311205 creator A5084602343 @default.
- W2083311205 creator A5084731978 @default.
- W2083311205 date "2008-01-01" @default.
- W2083311205 modified "2023-10-11" @default.
- W2083311205 title "Increased Static Pressure Promotes Migration of Vascular Smooth Muscle Cells: Involvement of the Rho-kinase Pathway" @default.
- W2083311205 cites W1501522779 @default.
- W2083311205 cites W1926488772 @default.
- W2083311205 cites W1971471876 @default.
- W2083311205 cites W1984561531 @default.
- W2083311205 cites W1988090831 @default.
- W2083311205 cites W1999983218 @default.
- W2083311205 cites W2000060365 @default.
- W2083311205 cites W2001485453 @default.
- W2083311205 cites W2001937608 @default.
- W2083311205 cites W2015843482 @default.
- W2083311205 cites W2019731805 @default.
- W2083311205 cites W2020993672 @default.
- W2083311205 cites W2025412540 @default.
- W2083311205 cites W2029231027 @default.
- W2083311205 cites W2042793531 @default.
- W2083311205 cites W2048249544 @default.
- W2083311205 cites W2048892415 @default.
- W2083311205 cites W2051292594 @default.
- W2083311205 cites W2052844291 @default.
- W2083311205 cites W2054411344 @default.
- W2083311205 cites W2065136678 @default.
- W2083311205 cites W2070710936 @default.
- W2083311205 cites W2072987324 @default.
- W2083311205 cites W2074416979 @default.
- W2083311205 cites W2078780479 @default.
- W2083311205 cites W2081543692 @default.
- W2083311205 cites W2083384274 @default.
- W2083311205 cites W2088041505 @default.
- W2083311205 cites W2096791952 @default.
- W2083311205 cites W2099225289 @default.
- W2083311205 cites W2100168858 @default.
- W2083311205 cites W2101907071 @default.
- W2083311205 cites W2103382643 @default.
- W2083311205 cites W2104485568 @default.
- W2083311205 cites W2114763381 @default.
- W2083311205 cites W2123988706 @default.
- W2083311205 cites W2133570574 @default.
- W2083311205 cites W2133846289 @default.
- W2083311205 cites W2134859355 @default.
- W2083311205 cites W2137570167 @default.
- W2083311205 cites W2138231208 @default.
- W2083311205 cites W2161595178 @default.
- W2083311205 cites W2164154096 @default.
- W2083311205 cites W2562329777 @default.
- W2083311205 cites W4213141530 @default.
- W2083311205 cites W4248973968 @default.
- W2083311205 cites W4376848265 @default.
- W2083311205 doi "https://doi.org/10.1097/fjc.0b013e31815b9d26" @default.
- W2083311205 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18209569" @default.
- W2083311205 hasPublicationYear "2008" @default.
- W2083311205 type Work @default.
- W2083311205 sameAs 2083311205 @default.
- W2083311205 citedByCount "17" @default.
- W2083311205 countsByYear W20833112052012 @default.
- W2083311205 countsByYear W20833112052014 @default.
- W2083311205 countsByYear W20833112052015 @default.
- W2083311205 countsByYear W20833112052016 @default.
- W2083311205 countsByYear W20833112052017 @default.
- W2083311205 countsByYear W20833112052019 @default.
- W2083311205 countsByYear W20833112052020 @default.
- W2083311205 countsByYear W20833112052022 @default.
- W2083311205 crossrefType "journal-article" @default.
- W2083311205 hasAuthorship W2083311205A5009988205 @default.
- W2083311205 hasAuthorship W2083311205A5012622166 @default.
- W2083311205 hasAuthorship W2083311205A5012657143 @default.
- W2083311205 hasAuthorship W2083311205A5021433405 @default.
- W2083311205 hasAuthorship W2083311205A5037302816 @default.
- W2083311205 hasAuthorship W2083311205A5058362593 @default.
- W2083311205 hasAuthorship W2083311205A5062835440 @default.
- W2083311205 hasAuthorship W2083311205A5084602343 @default.
- W2083311205 hasAuthorship W2083311205A5084731978 @default.
- W2083311205 hasBestOaLocation W20833112051 @default.
- W2083311205 hasConcept C126322002 @default.
- W2083311205 hasConcept C134018914 @default.
- W2083311205 hasConcept C1491633281 @default.
- W2083311205 hasConcept C164144092 @default.
- W2083311205 hasConcept C184235292 @default.
- W2083311205 hasConcept C185592680 @default.
- W2083311205 hasConcept C195687474 @default.
- W2083311205 hasConcept C201800478 @default.
- W2083311205 hasConcept C2779395532 @default.
- W2083311205 hasConcept C2992686903 @default.
- W2083311205 hasConcept C55493867 @default.
- W2083311205 hasConcept C71924100 @default.