FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083314535> ?p ?o ?g. }

• W2083314535 endingPage "1032" @default.
• W2083314535 startingPage "1025" @default.
• W2083314535 abstract "We report a novel method for targeting adenovirus-mediated gene delivery. By irradiating mammalian cells prior to adenoviral transduction, adenoviral gene transfer is greatly improved and the adenoviral genome integrates into cellular DNA. In this work, human and rodent cell lines were irradiated and subsequently transduced with the adenovirus vector Ad5CMVlacZ. Initial levels of transduction were as much as 40-fold higher in irradiated cells, and this improvement in transduction was radiation dose dependent. The duration of lacZ expression in irradiated cells was also much longer than in nonirradiated cells and reached a plateau after 21 days. At doses of 7 Gy, long-term (<50 day) expression of lacZ could be detected in 15% of cells by flow cytometry. This long-lasting expression of lacZ was due to viral DNA integration into the host genome. Thus, pretreatment of cells with ionizing radiation improves both immediate transduction efficiency and duration of transgene expression. This may lead to the development of new protocols combining radiation and gene therapy in treating human malignancy. We show that modest doses of ionizing radiation can greatly improve adenoviral transduction efficiency. This improvement of gene transfer was found in mouse (NIH-3T3) cells, human neoplastic cells (A549), and primary human cells (39F). This improvement is long lasting (more than 3 weeks). This long-lasting expression in irradiated cells is due to viral DNA integration into the host genome. The combination of radiation with adenoviral transduction increases both initial and long-term viral transgene expression, and may lead to new combined modality protocols." @default.
• W2083314535 created "2016-06-24" @default.
• W2083314535 creator A5002125828 @default.
• W2083314535 creator A5010322434 @default.
• W2083314535 creator A5012698759 @default.
• W2083314535 creator A5055888843 @default.
• W2083314535 date "1997-06-10" @default.
• W2083314535 modified "2023-09-27" @default.
• W2083314535 title "High-Efficiency Stable Gene Transfer of Adenovirus into Mammalian Cells Using Ionizing Radiation" @default.
• W2083314535 cites W1966669660 @default.
• W2083314535 cites W1967937424 @default.
• W2083314535 cites W1978542226 @default.
• W2083314535 cites W1990545247 @default.
• W2083314535 cites W1993298310 @default.
• W2083314535 cites W1999157369 @default.
• W2083314535 cites W2006710343 @default.
• W2083314535 cites W2009044337 @default.
• W2083314535 cites W2011229774 @default.
• W2083314535 cites W2021783595 @default.
• W2083314535 cites W2024356087 @default.
• W2083314535 cites W2026211946 @default.
• W2083314535 cites W2029812891 @default.
• W2083314535 cites W2033516066 @default.
• W2083314535 cites W2053944961 @default.
• W2083314535 cites W2060289254 @default.
• W2083314535 cites W2062363361 @default.
• W2083314535 cites W2067587032 @default.
• W2083314535 cites W2067861173 @default.
• W2083314535 cites W2077231978 @default.
• W2083314535 cites W2078321284 @default.
• W2083314535 cites W2081262189 @default.
• W2083314535 cites W2083457187 @default.
• W2083314535 cites W2086584640 @default.
• W2083314535 cites W2092127983 @default.
• W2083314535 cites W2110393059 @default.
• W2083314535 cites W2114689164 @default.
• W2083314535 cites W2151902657 @default.
• W2083314535 cites W2158744232 @default.
• W2083314535 cites W2313269464 @default.
• W2083314535 cites W945876882 @default.
• W2083314535 doi "https://doi.org/10.1089/hum.1997.8.9-1025" @default.
• W2083314535 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9189760" @default.
• W2083314535 hasPublicationYear "1997" @default.
• W2083314535 type Work @default.
• W2083314535 sameAs 2083314535 @default.
• W2083314535 citedByCount "68" @default.
• W2083314535 countsByYear W20833145352012 @default.
• W2083314535 countsByYear W20833145352014 @default.
• W2083314535 countsByYear W20833145352017 @default.
• W2083314535 countsByYear W20833145352019 @default.
• W2083314535 countsByYear W20833145352020 @default.
• W2083314535 countsByYear W20833145352022 @default.
• W2083314535 crossrefType "journal-article" @default.
• W2083314535 hasAuthorship W2083314535A5002125828 @default.
• W2083314535 hasAuthorship W2083314535A5010322434 @default.
• W2083314535 hasAuthorship W2083314535A5012698759 @default.
• W2083314535 hasAuthorship W2083314535A5055888843 @default.
• W2083314535 hasConcept C102230213 @default.
• W2083314535 hasConcept C104317684 @default.
• W2083314535 hasConcept C111599444 @default.
• W2083314535 hasConcept C135983454 @default.
• W2083314535 hasConcept C150194340 @default.
• W2083314535 hasConcept C15152581 @default.
• W2083314535 hasConcept C153911025 @default.
• W2083314535 hasConcept C2776954459 @default.
• W2083314535 hasConcept C32470452 @default.
• W2083314535 hasConcept C40767141 @default.
• W2083314535 hasConcept C502942594 @default.
• W2083314535 hasConcept C54355233 @default.
• W2083314535 hasConcept C55493867 @default.
• W2083314535 hasConcept C81885089 @default.
• W2083314535 hasConcept C86803240 @default.
• W2083314535 hasConcept C95444343 @default.
• W2083314535 hasConceptScore W2083314535C102230213 @default.
• W2083314535 hasConceptScore W2083314535C104317684 @default.
• W2083314535 hasConceptScore W2083314535C111599444 @default.
• W2083314535 hasConceptScore W2083314535C135983454 @default.
• W2083314535 hasConceptScore W2083314535C150194340 @default.
• W2083314535 hasConceptScore W2083314535C15152581 @default.
• W2083314535 hasConceptScore W2083314535C153911025 @default.
• W2083314535 hasConceptScore W2083314535C2776954459 @default.
• W2083314535 hasConceptScore W2083314535C32470452 @default.
• W2083314535 hasConceptScore W2083314535C40767141 @default.
• W2083314535 hasConceptScore W2083314535C502942594 @default.
• W2083314535 hasConceptScore W2083314535C54355233 @default.
• W2083314535 hasConceptScore W2083314535C55493867 @default.
• W2083314535 hasConceptScore W2083314535C81885089 @default.
• W2083314535 hasConceptScore W2083314535C86803240 @default.
• W2083314535 hasConceptScore W2083314535C95444343 @default.
• W2083314535 hasIssue "9" @default.
• W2083314535 hasLocation W20833145351 @default.
• W2083314535 hasLocation W20833145352 @default.
• W2083314535 hasOpenAccess W2083314535 @default.
• W2083314535 hasPrimaryLocation W20833145351 @default.
• W2083314535 hasRelatedWork W1981864943 @default.
• W2083314535 hasRelatedWork W1986851889 @default.
• W2083314535 hasRelatedWork W1994284515 @default.
• W2083314535 hasRelatedWork W1998100163 @default.

• next