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• W2083315745 abstract "Entomopathogenic nematodes are widely used as biological control agents that can suppress or evade the host immune defense upon entry into insects. The surface coat of Steinernema glaseri has been shown to play important roles in defeating the host immune system. In this work, a protein fraction with antiphagocytic activity was separated by electro-elution and further analyzed by two-dimensional electrophoresis (2-DE). LC–MS/MS analysis of one protein spot from a 2-DE gel gave five peptides that were highly similar to enolases of many organisms. A 1311 bp cDNA was cloned that encodes a 47 kDa protein with high sequence identity to enolases from different species of nematodes. The deduced protein, Sg-ENOL, was expressed in Escherichia coli, and its glycolytic activity was demonstrated by the conversion of 2-phospho-d-glycerate to phosphoenolpyruvate. Recombinant Sg-ENOL significantly reduced the LT50s of Xenorhabdus poinarii and Metarhizium anisopliae when co-injected into Galleria mellonella and Locusta migratoria manilensis Meyen, respectively. Using immuno-gold transmission electron microscopy, native Sg-ENOL was confirmed to be localized to both the nematode cuticle and the surface coat. In vitro, secretion of Sg-ENOL was inducible rather than constitutive. In vivo, Sg-ENOL was detected in the host hemolymph after infection of G. mellonella with S. glaseri, indicating that Sg-ENOL was secreted into the insect hemocoel and was involved in infection. This is the first report of the cloning and characterization of a surface coat protein in an entomopathogenic nematode. Our findings provide clear evidence for an important role for a cell surface enolase in S. glaseri infection and host immune suppression." @default.
• W2083315745 created "2016-06-24" @default.
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• W2083315745 date "2012-10-01" @default.
• W2083315745 modified "2023-10-14" @default.
• W2083315745 title "Steinernema glaseri surface enolase: Molecular cloning, biological characterization, and role in host immune suppression" @default.
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• W2083315745 doi "https://doi.org/10.1016/j.molbiopara.2012.06.006" @default.
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