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- W2083359500 abstract "The expression of HLA-C on the surface of platelets is rarely studied due to the lack of proper alloantisera. We addressed this question using an IgM human monoclonal antibody directed against HLA-Cw1 (VP6G3).Both flow cytometry and complement dependent cytotoxicity studies were used in the current analysis.The expression of the HLA-Cw1 antigen on platelets is lower than on peripheral blood lymphocytes as shown by flow cytometry. Variation in expression levels between individuals is also observed. Using this antibody in a complement-dependent cytotoxicity assay, we did not observe lysis using platelets as targets, whereas peripheral blood lymphocytes of the same blood donors were adequately lysed.These results confirm that platelets indeed express HLA-C. Furthermore, the results support the insignificant role of HLA-C in immunological platelet refractoriness." @default.
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- W2083359500 date "2000-01-01" @default.
- W2083359500 modified "2023-09-22" @default.
- W2083359500 title "HLA-C Expression on Platelets: Studies with an HLA-Cw1-Specific Human Monoclonal Antibody" @default.
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- W2083359500 doi "https://doi.org/10.1159/000031221" @default.
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