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• W2083360839 abstract "We have previously demonstrated a portable biosensor that utilizes networks of mammalian neurons on microelectrode arrays (MEAs) as the sensing element. These neuronal cultures on MEAs are derived from primary neuronal tissues and are short-lived. In order to extend the shelf life of neuronal networks for use in a fieldable sensor technology, a renewable source of networks is needed. Neural stem and progenitor cells are capable of self-renewal and differentiation into functional neuronal networks. The purpose of this study was to develop a strategy for growing passaged neural stem and progenitor cells on MEAs under controlled conditions to produce differentiated neurons and glia comprising functional neuronal networks. Primary and passaged neuroepithelial stem and progenitor cells dissociated from embryonic day 13 rat cortex were seeded on MEAs and maintained with serum-free medium containing basic fibroblast growth factor (bFGF) combined with brain-derived neurotrophic factor (BDNF). These culture conditions lead to abundant neurons, with astrocytes as supportive cells, forming synaptically linked networks of neurons. Spontaneous action potentials were best recorded from networks derived from primary or passaged progenitor cells 4–5 weeks after initial culture. The passaged progenitor cell-derived networks on MEAs responded to the GABAA antagonist bicuculline, the NMDA glutamate inhibitor APV, and the non-NMDA glutamate antagonist CNQX indicating active synapses were present. Passaged neural stem and progenitor cell-derived networks on MEAs have properties similar to networks derived from primary neuronal cultures and can serve as a renewable supply of sensor elements for detection of environmental threats." @default.
• W2083360839 created "2016-06-24" @default.
• W2083360839 creator A5053464028 @default.
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• W2083360839 date "2009-04-01" @default.
• W2083360839 modified "2023-09-25" @default.
• W2083360839 title "Passaged neural stem cell-derived neuronal networks for a portable biosensor" @default.
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• W2083360839 doi "https://doi.org/10.1016/j.bios.2008.12.007" @default.
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